An unconventional enzyme-immunoassay for the evaluation of antigenic potency of bacterial extracts

C. Peri, C. Pizzimenti, V. Folisi, F. Molina, G. Cangemi, G. Melioli

Research output: Contribution to journalArticle

Abstract

Background. The evaluation of the antigen concentration of bacterial vaccines is crucial both on raw materials and in final products, such as drugs. Bacterial lysates are used in humans to improve the immune response during upper respiratory tract infections. Methods. An enzyme immunoassay, which employs a single specific antiserum and suboptimal concentration of antigen coated to plastic wells, was developed to evaluate the concentration of antigens in Branhamella catarrhalis lysates. Results. Sensitivity was good with detection of only a few nanograms of antigen, and better than that of the rocket electrophoresis used as the reference method. The assay was highly reproducible both in intra-day and between-day experiments. For this reason, it is suitable for use both in routine quality control and during the research and development of new antigenic products. Conclusions. The control of the production process of infectious agent-derived antigens in the pharmacological industry could be significantly improved using this low-cost, easily automated assay.

Original languageEnglish
Pages (from-to)179-184
Number of pages6
JournalMinerva Biotecnologica
Volume15
Issue number3
Publication statusPublished - Sep 2003

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Antigens
Immunoenzyme Techniques
Enzymes
Assays
Bacterial Vaccines
Moraxella (Branhamella) catarrhalis
Rockets
Electrophoresis
Vaccines
Quality control
Immune Sera
Raw materials
Respiratory Tract Infections
Quality Control
Plastics
Industry
Pharmacology
Costs and Cost Analysis
Pharmaceutical Preparations
Costs

Keywords

  • Branhamella catarrhalis
  • Enzyme-immunoassay
  • Lysates

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Peri, C., Pizzimenti, C., Folisi, V., Molina, F., Cangemi, G., & Melioli, G. (2003). An unconventional enzyme-immunoassay for the evaluation of antigenic potency of bacterial extracts. Minerva Biotecnologica, 15(3), 179-184.

An unconventional enzyme-immunoassay for the evaluation of antigenic potency of bacterial extracts. / Peri, C.; Pizzimenti, C.; Folisi, V.; Molina, F.; Cangemi, G.; Melioli, G.

In: Minerva Biotecnologica, Vol. 15, No. 3, 09.2003, p. 179-184.

Research output: Contribution to journalArticle

Peri, C, Pizzimenti, C, Folisi, V, Molina, F, Cangemi, G & Melioli, G 2003, 'An unconventional enzyme-immunoassay for the evaluation of antigenic potency of bacterial extracts', Minerva Biotecnologica, vol. 15, no. 3, pp. 179-184.
Peri, C. ; Pizzimenti, C. ; Folisi, V. ; Molina, F. ; Cangemi, G. ; Melioli, G. / An unconventional enzyme-immunoassay for the evaluation of antigenic potency of bacterial extracts. In: Minerva Biotecnologica. 2003 ; Vol. 15, No. 3. pp. 179-184.
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