Abstract
The aim of this article is to review a number of variables that may affect the cryopreservation of minimally manipulated products containing allogeneic or autologous hemopoietic progenitor cells (HPC) used for transplantation, with particular reference to processing, type and addition of cryoprotectant, cell concentration, volume, freezing procedure, cooling rate, storage, thawing, and quality management. After defining final product's requirements in compliance with norms, laws and regulations, it is crucial to define the critical control points of the process.New approaches of processing were developed in the last few years such as automatic devices for volume reduction and high cell concentration in the frozen product. DMSO at 10% final concentration is still the most used cryoprotectant for HPC cryopreservation. Although controlled rate freezing is the recommended method for HPC cryopreservation, alternative methods may be used. Last generation vapor storage vessels ensure temperature stability better than older tanks. Their use may reduce risks of cross-contamination.Finally we review advantages and disadvantages of thawing procedures that may be carried out in the laboratory or at the patient's bedside.
| Original language | English |
|---|---|
| Pages (from-to) | 324-336 |
| Number of pages | 13 |
| Journal | Transfusion and Apheresis Science |
| Volume | 54 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jun 1 2016 |
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Keywords
- Cryopreservation
- Hemopoietic stem cells
- Thawing
ASJC Scopus subject areas
- Hematology
Cite this
An update on methods for cryopreservation and thawing of hemopoietic stem cells. / Lecchi, Lucilla; Giovanelli, Silvia; Gagliardi, Barbara; Pezzali, Ilaria; Ratti, Ilaria; Marconi, Maurizio.
In: Transfusion and Apheresis Science, Vol. 54, No. 3, 01.06.2016, p. 324-336.Research output: Contribution to journal › Review article
}
TY - JOUR
T1 - An update on methods for cryopreservation and thawing of hemopoietic stem cells
AU - Lecchi, Lucilla
AU - Giovanelli, Silvia
AU - Gagliardi, Barbara
AU - Pezzali, Ilaria
AU - Ratti, Ilaria
AU - Marconi, Maurizio
PY - 2016/6/1
Y1 - 2016/6/1
N2 - The aim of this article is to review a number of variables that may affect the cryopreservation of minimally manipulated products containing allogeneic or autologous hemopoietic progenitor cells (HPC) used for transplantation, with particular reference to processing, type and addition of cryoprotectant, cell concentration, volume, freezing procedure, cooling rate, storage, thawing, and quality management. After defining final product's requirements in compliance with norms, laws and regulations, it is crucial to define the critical control points of the process.New approaches of processing were developed in the last few years such as automatic devices for volume reduction and high cell concentration in the frozen product. DMSO at 10% final concentration is still the most used cryoprotectant for HPC cryopreservation. Although controlled rate freezing is the recommended method for HPC cryopreservation, alternative methods may be used. Last generation vapor storage vessels ensure temperature stability better than older tanks. Their use may reduce risks of cross-contamination.Finally we review advantages and disadvantages of thawing procedures that may be carried out in the laboratory or at the patient's bedside.
AB - The aim of this article is to review a number of variables that may affect the cryopreservation of minimally manipulated products containing allogeneic or autologous hemopoietic progenitor cells (HPC) used for transplantation, with particular reference to processing, type and addition of cryoprotectant, cell concentration, volume, freezing procedure, cooling rate, storage, thawing, and quality management. After defining final product's requirements in compliance with norms, laws and regulations, it is crucial to define the critical control points of the process.New approaches of processing were developed in the last few years such as automatic devices for volume reduction and high cell concentration in the frozen product. DMSO at 10% final concentration is still the most used cryoprotectant for HPC cryopreservation. Although controlled rate freezing is the recommended method for HPC cryopreservation, alternative methods may be used. Last generation vapor storage vessels ensure temperature stability better than older tanks. Their use may reduce risks of cross-contamination.Finally we review advantages and disadvantages of thawing procedures that may be carried out in the laboratory or at the patient's bedside.
KW - Cryopreservation
KW - Hemopoietic stem cells
KW - Thawing
UR - http://www.scopus.com/inward/record.url?scp=84971372987&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84971372987&partnerID=8YFLogxK
U2 - 10.1016/j.transci.2016.05.009
DO - 10.1016/j.transci.2016.05.009
M3 - Review article
VL - 54
SP - 324
EP - 336
JO - Transfusion and Apheresis Science
JF - Transfusion and Apheresis Science
SN - 1473-0502
IS - 3
ER -