TY - JOUR
T1 - An upstream negative regulatory element in human granulocyte-macrophage colony-stimulating factor promoter is recognised by AP1 family members
AU - Gramigni, Claudia
AU - Penco, Silvana
AU - Bianchi-Scarrà, Giovanna
AU - Ravazzolo, Roberto
AU - Garrè, Cecilia
PY - 1998/11/27
Y1 - 1998/11/27
N2 - Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine involved in haematopoiesis and host defence. Production of GM-CSF has been detected in tumour cells including the U87MG astrocytoma cell line. Previous studies have been focused on the regulatory role of the proximal region of the GM-CSF promoter. Our studies on the distal region of the promoter in U87MG cells identify a negative cis element (-1377/-1298) which contains a AP1-like site able to bind c-jun and c-fos transcription factors, according to the results of DNA/protein binding assays. Mutagenesis of the AP1-like site eliminates AP1 binding and the negative effect on promoter activity. Copyright (C) 1998 Federation of European Biochemical Societies.
AB - Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine involved in haematopoiesis and host defence. Production of GM-CSF has been detected in tumour cells including the U87MG astrocytoma cell line. Previous studies have been focused on the regulatory role of the proximal region of the GM-CSF promoter. Our studies on the distal region of the promoter in U87MG cells identify a negative cis element (-1377/-1298) which contains a AP1-like site able to bind c-jun and c-fos transcription factors, according to the results of DNA/protein binding assays. Mutagenesis of the AP1-like site eliminates AP1 binding and the negative effect on promoter activity. Copyright (C) 1998 Federation of European Biochemical Societies.
KW - AP1
KW - Granulocyte-macrophage colony-stimulating factor
KW - Negative cis element
KW - Transcriptional regulation
KW - U87MG
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U2 - 10.1016/S0014-5793(98)01443-4
DO - 10.1016/S0014-5793(98)01443-4
M3 - Article
C2 - 9862439
AN - SCOPUS:0032573401
VL - 440
SP - 119
EP - 124
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1-2
ER -