Abstract
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine involved in haematopoiesis and host defence. Production of GM-CSF has been detected in tumour cells including the U87MG astrocytoma cell line. Previous studies have been focused on the regulatory role of the proximal region of the GM-CSF promoter. Our studies on the distal region of the promoter in U87MG cells identify a negative cis element (-1377/-1298) which contains a AP1-like site able to bind c-jun and c-fos transcription factors, according to the results of DNA/protein binding assays. Mutagenesis of the AP1-like site eliminates AP1 binding and the negative effect on promoter activity. Copyright (C) 1998 Federation of European Biochemical Societies.
| Original language | English |
|---|---|
| Pages (from-to) | 119-124 |
| Number of pages | 6 |
| Journal | FEBS Letters |
| Volume | 440 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Nov 27 1998 |
Keywords
- AP1
- Granulocyte-macrophage colony-stimulating factor
- Negative cis element
- Transcriptional regulation
- U87MG
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Fingerprint Dive into the research topics of 'An upstream negative regulatory element in human granulocyte-macrophage colony-stimulating factor promoter is recognised by AP1 family members'. Together they form a unique fingerprint.
Cite this
Gramigni, C., Penco, S., Bianchi-Scarrà, G., Ravazzolo, R., & Garrè, C. (1998). An upstream negative regulatory element in human granulocyte-macrophage colony-stimulating factor promoter is recognised by AP1 family members. FEBS Letters, 440(1-2), 119-124. https://doi.org/10.1016/S0014-5793(98)01443-4