An upstream negative regulatory element in human granulocyte-macrophage colony-stimulating factor promoter is recognised by AP1 family members

Claudia Gramigni; Silvana Penco; Giovanna Bianchi-Scarrà; Roberto Ravazzolo; Cecilia Garrè

doi:10.1016/S0014-5793(98)01443-4

An upstream negative regulatory element in human granulocyte-macrophage colony-stimulating factor promoter is recognised by AP1 family members

Claudia Gramigni, Silvana Penco, Giovanna Bianchi-Scarrà, Roberto Ravazzolo, Cecilia Garrè

Research output: Contribution to journal › Article

Abstract

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine involved in haematopoiesis and host defence. Production of GM-CSF has been detected in tumour cells including the U87MG astrocytoma cell line. Previous studies have been focused on the regulatory role of the proximal region of the GM-CSF promoter. Our studies on the distal region of the promoter in U87MG cells identify a negative cis element (-1377/-1298) which contains a AP1-like site able to bind c-jun and c-fos transcription factors, according to the results of DNA/protein binding assays. Mutagenesis of the AP1-like site eliminates AP1 binding and the negative effect on promoter activity. Copyright (C) 1998 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	119-124
Number of pages	6
Journal	FEBS Letters
Volume	440
Issue number	1-2
DOIs	https://doi.org/10.1016/S0014-5793(98)01443-4
Publication status	Published - Nov 27 1998

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Keywords

AP1
Granulocyte-macrophage colony-stimulating factor
Negative cis element
Transcriptional regulation
U87MG

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

Cite this

Gramigni, C., Penco, S., Bianchi-Scarrà, G., Ravazzolo, R., & Garrè, C. (1998). An upstream negative regulatory element in human granulocyte-macrophage colony-stimulating factor promoter is recognised by AP1 family members. FEBS Letters, 440(1-2), 119-124. https://doi.org/10.1016/S0014-5793(98)01443-4

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10.1016/S0014-5793(98)01443-4