An upstream positive regulatory element in human GM-CSF promoter is recognized by NF-κB/Rel family members

Marco Musso, Paola Ghiorzo, Paola Fiorentini, Roberto Giuffrida, Paola Ciotti, Cecilia Garré, Roberto Ravazzolo, Giovanna Bianchi-Scarrà

Research output: Contribution to journalArticlepeer-review

Abstract

To further extend the previous analysis of cis-acting elements and cognate trans-acting factors that contribute to GM-CSF transcriptional regulation, we have examined a promoter region between -1742 and -2010. DNase I footprinting assays showed four protected sequences named A, B, C and D. DNA transfections in the T-lymphoid Mo cell line, which constitutively expresses GM-CSF, indicated that the A element, located between -2002 and -1984, has a positive role on transcription. Further characterization by electrophoretic mobility shift assays in the presence of different competitor oligonucleotides showed that this element binds a factor of the NF-κB/Rel family.

Original languageEnglish
Pages (from-to)64-72
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume223
Issue number1
DOIs
Publication statusPublished - Jun 5 1996

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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