An upstream positive regulatory element in human GM-CSF promoter is recognized by NF-κB/Rel family members

Marco Musso; Paola Ghiorzo; Paola Fiorentini; Roberto Giuffrida; Paola Ciotti; Cecilia Garré; Roberto Ravazzolo; Giovanna Bianchi-Scarrà

doi:10.1006/bbrc.1996.0847

An upstream positive regulatory element in human GM-CSF promoter is recognized by NF-κB/Rel family members

Marco Musso, Paola Ghiorzo, Paola Fiorentini, Roberto Giuffrida, Paola Ciotti, Cecilia Garré, Roberto Ravazzolo, Giovanna Bianchi-Scarrà

Research output: Contribution to journal › Article › peer-review

Abstract

To further extend the previous analysis of cis-acting elements and cognate trans-acting factors that contribute to GM-CSF transcriptional regulation, we have examined a promoter region between -1742 and -2010. DNase I footprinting assays showed four protected sequences named A, B, C and D. DNA transfections in the T-lymphoid Mo cell line, which constitutively expresses GM-CSF, indicated that the A element, located between -2002 and -1984, has a positive role on transcription. Further characterization by electrophoretic mobility shift assays in the presence of different competitor oligonucleotides showed that this element binds a factor of the NF-κB/Rel family.

Original language	English
Pages (from-to)	64-72
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	223
Issue number	1
DOIs	https://doi.org/10.1006/bbrc.1996.0847
Publication status	Published - Jun 5 1996

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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An upstream positive regulatory element in human GM-CSF promoter is recognized by NF-κB/Rel family members. / Musso, Marco; Ghiorzo, Paola; Fiorentini, Paola; Giuffrida, Roberto; Ciotti, Paola; Garré, Cecilia; Ravazzolo, Roberto; Bianchi-Scarrà, Giovanna.

In: Biochemical and Biophysical Research Communications, Vol. 223, No. 1, 05.06.1996, p. 64-72.

Research output: Contribution to journal › Article › peer-review

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