TY - JOUR
T1 - An upstream positive regulatory element in human GM-CSF promoter is recognized by NF-κB/Rel family members
AU - Musso, Marco
AU - Ghiorzo, Paola
AU - Fiorentini, Paola
AU - Giuffrida, Roberto
AU - Ciotti, Paola
AU - Garré, Cecilia
AU - Ravazzolo, Roberto
AU - Bianchi-Scarrà, Giovanna
PY - 1996/6/5
Y1 - 1996/6/5
N2 - To further extend the previous analysis of cis-acting elements and cognate trans-acting factors that contribute to GM-CSF transcriptional regulation, we have examined a promoter region between -1742 and -2010. DNase I footprinting assays showed four protected sequences named A, B, C and D. DNA transfections in the T-lymphoid Mo cell line, which constitutively expresses GM-CSF, indicated that the A element, located between -2002 and -1984, has a positive role on transcription. Further characterization by electrophoretic mobility shift assays in the presence of different competitor oligonucleotides showed that this element binds a factor of the NF-κB/Rel family.
AB - To further extend the previous analysis of cis-acting elements and cognate trans-acting factors that contribute to GM-CSF transcriptional regulation, we have examined a promoter region between -1742 and -2010. DNase I footprinting assays showed four protected sequences named A, B, C and D. DNA transfections in the T-lymphoid Mo cell line, which constitutively expresses GM-CSF, indicated that the A element, located between -2002 and -1984, has a positive role on transcription. Further characterization by electrophoretic mobility shift assays in the presence of different competitor oligonucleotides showed that this element binds a factor of the NF-κB/Rel family.
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U2 - 10.1006/bbrc.1996.0847
DO - 10.1006/bbrc.1996.0847
M3 - Article
C2 - 8660380
AN - SCOPUS:0029982425
VL - 223
SP - 64
EP - 72
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -