Analogs of the Golgi complex in microsporidia: Structure and avesicular mechanisms of function

Galina V. Beznoussenko, Viacheslav V. Dolgikh, Elena V. Seliverstova, Petr B. Semenov, Yuri S. Tokarev, Alvar Trucco, Massimo Micaroni, Daniele Di Giandomenico, Peter Auinger, Igor V. Senderskly, Sergei O. Skarlato, Ekaterina S. Snigirevskaya, Yan Yu Komissarchik, Margit Pavelka, Maria A. De Matteis, Alberto Luini, Yuliya Ya Sokolova, Alexander A. Mironov

Research output: Contribution to journalArticle

Abstract

Microsporidia are obligatory intracellular parasites, most species of which live in the host cell cytosol. They synthesize and then transport secretory proteins from the endoplasmic reticulum to the plasma membrane for formation of the spore wall and the polar tube for cell invasion. However, microsporidia do not have a typical Golgi complex. Here, using quick-freezing cryosubstitution and chemical fixation, we demonstrate that the Golgi analogs of the microsporidia Paranosema (Antonospora) grylli and Paranosema locustae appear as 300-nm networks of thin (25- to 40-mn diameter), branching or varicose tubules that display histochemical features of a Golgi, but that do not have vesicles. Vesicles are not formed even if membrane fusion is inhibited. These tubular networks are connected to the endoplasmic reticulum, the plasma membrane and the forming polar tube, and are positive for Sec13, γCOP and analogs of giantin and GM130. The spore-wall and polar-tube proteins are transported from the endoplasmic reticulum to the target membranes through these tubular networks, within which they undergo concentration and glycosylation. We suggest that the intracellular transport of secreted proteins in microsporidia occurs by a progression mechanism that does not involve the participation of vesicles generated by coat proteins I and II.

Original languageEnglish
Pages (from-to)1288-1298
Number of pages11
JournalJournal of Cell Science
Volume120
Issue number7
DOIs
Publication statusPublished - Apr 1 2007

Keywords

  • COP-I vesicles
  • Golgi
  • Intracellular transport
  • Microsporidia

ASJC Scopus subject areas

  • Cell Biology

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    Beznoussenko, G. V., Dolgikh, V. V., Seliverstova, E. V., Semenov, P. B., Tokarev, Y. S., Trucco, A., Micaroni, M., Di Giandomenico, D., Auinger, P., Senderskly, I. V., Skarlato, S. O., Snigirevskaya, E. S., Komissarchik, Y. Y., Pavelka, M., De Matteis, M. A., Luini, A., Sokolova, Y. Y., & Mironov, A. A. (2007). Analogs of the Golgi complex in microsporidia: Structure and avesicular mechanisms of function. Journal of Cell Science, 120(7), 1288-1298. https://doi.org/10.1242/jcs.03402