Analysis and significance of anti-latent membrane protein-1 antibodies in the sera of patients with EBV-associated diseases

Jingwu Xu, Ali Ahmad, Mario D'Addario, Laurent Knafo, James F. Jones, U. Prasad, R. Dolcetti, E. Vaccher, José Menezes

Research output: Contribution to journalArticle

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Abstract

Anti-latent membrane protein-1 (LMP-1) is an EBV-encoded type III integral membrane protein with oncogenic potential that is expressed most consistently in various EBV-associated malignancies. Unlike many other EBV proteins, LMP-1 Abs have rarely been demonstrated in EBV-associated disease conditions. We established a high level LMP-1-expressing cell clone and used it for the detection, quantitation, and characterization of these Abs in various human sera in immunoblots and ELISA. Our results demonstrate that, in contrast to the commonly held notion, LMP-1 induces significant humoral immune responses in EBV-associated malignant conditions especially in nasopharyngeal carcinoma (NPC) patients in whom >70% sera are positive for these Abs, and their titers correlate with the clinical condition of the tumors. Interestingly, anti-LMP-1 Abs of IgA isotype were found only in NPC patients. These Abs were absent from the sera of infectious mononucleosis and chronic EBV infection patients, whereas a small fraction (~5%) of the healthy, EBV-seropositive individuals were positive for them; however, their OD values were much lower than those of NPC patients. These studies demonstrate, for the first time, the potential significance of LMP-1-specific Abs for the diagnosis and prognosis of EBV-associated malignancies, especially of NPC.

Original languageEnglish
Pages (from-to)2815-2822
Number of pages8
JournalJournal of Immunology
Volume164
Issue number5
Publication statusPublished - Mar 1 2000

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Human Herpesvirus 4
Membrane Proteins
Antibodies
Serum
Neoplasms
Infectious Mononucleosis
Epstein-Barr Virus Infections
Humoral Immunity
Immunoglobulin A
Clone Cells
Enzyme-Linked Immunosorbent Assay
Nasopharyngeal carcinoma
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Xu, J., Ahmad, A., D'Addario, M., Knafo, L., Jones, J. F., Prasad, U., ... Menezes, J. (2000). Analysis and significance of anti-latent membrane protein-1 antibodies in the sera of patients with EBV-associated diseases. Journal of Immunology, 164(5), 2815-2822.

Analysis and significance of anti-latent membrane protein-1 antibodies in the sera of patients with EBV-associated diseases. / Xu, Jingwu; Ahmad, Ali; D'Addario, Mario; Knafo, Laurent; Jones, James F.; Prasad, U.; Dolcetti, R.; Vaccher, E.; Menezes, José.

In: Journal of Immunology, Vol. 164, No. 5, 01.03.2000, p. 2815-2822.

Research output: Contribution to journalArticle

Xu, J, Ahmad, A, D'Addario, M, Knafo, L, Jones, JF, Prasad, U, Dolcetti, R, Vaccher, E & Menezes, J 2000, 'Analysis and significance of anti-latent membrane protein-1 antibodies in the sera of patients with EBV-associated diseases', Journal of Immunology, vol. 164, no. 5, pp. 2815-2822.
Xu, Jingwu ; Ahmad, Ali ; D'Addario, Mario ; Knafo, Laurent ; Jones, James F. ; Prasad, U. ; Dolcetti, R. ; Vaccher, E. ; Menezes, José. / Analysis and significance of anti-latent membrane protein-1 antibodies in the sera of patients with EBV-associated diseases. In: Journal of Immunology. 2000 ; Vol. 164, No. 5. pp. 2815-2822.
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