Analysis of 4-ABP-DNA adducts and p53 alterations in urinary bladder carcinoma

Gianpiero Romano, Lorella Garagnani, Alma Boninsegna, Paolo Ferrari, Giovanna Flamini, Cristina De Gaetani, Alessandro Sgambato, Ferrari Giovanni, Giuseppe Curigliano, Gianluigi Ferretti, Achille Cittadini, Gianpaolo Trentini

Research output: Contribution to journalArticle

Abstract

Background: Activated intermediates of 4-aminobiphenyl (4-ABP) are able to covalently interact with DNA to form adducts. There is a large body of evidence indicating that carcinogen-DNA adduct formation can be one of the cancer initiating mechanisms. Materials and Methods: (4-ABP)-induced DNA damage in association with p53 overexpression and mutations were evaluated in specimens of urothelial bladder cancer from 106 patients. Results: 4-ABP-DNA adduct levels resulted higher in smokers compared to non smokers, with a borderline statistical value. p53 nuclear overexpression was related to tumor grading, while no significant correlation with stage, 4-ABP-DNA adducts, smoking habit, and disease recurrence could be observed. Concerning molecular analysis, p53 point mutations were found in 17 of 106 cases (16%) and mutational pattern was significantly associated both with higher grade and stage, but no correlation was found with disease recurrence. Conclusion: These results suggest that other sources, in addition to tobacco smoke, may contribute to 4-ABP-DNA adducts formation in bladder tissue and that p53 expression/mutation cannot be considered a prognostic factor in bladder cancer.

Original languageEnglish
Pages (from-to)4571-4576
Number of pages6
JournalAnticancer Research
Volume19
Issue number5 C
Publication statusPublished - 1999

Keywords

  • 4-ABP-DNA adducts
  • Bladder cancer
  • Cigarette smoke
  • Molecular epidemiology
  • p53 overexpression/mutations

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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  • Cite this

    Romano, G., Garagnani, L., Boninsegna, A., Ferrari, P., Flamini, G., De Gaetani, C., Sgambato, A., Giovanni, F., Curigliano, G., Ferretti, G., Cittadini, A., & Trentini, G. (1999). Analysis of 4-ABP-DNA adducts and p53 alterations in urinary bladder carcinoma. Anticancer Research, 19(5 C), 4571-4576.