Analysis of 5-pyrrolidone-2-carboxylate ester by reverse phase high-performance liquid chromatography

E. Bousquet, V. Guarcello, M. C. Morale, V. Rizza

Research output: Contribution to journalArticlepeer-review

Abstract

A simple quantitative method for estimating nanomole concentrations of 5-pyrrolidone-2-carboxylic acid (PCA) in tissue homogenates from mouse has been developed using reversephase HPLC. PCA was detected as the 4-nitrophenacyl ester which has an absorption maximum at 263 nm, a relatively high stability, and excellent chromatographic separation and detectability. This method offers distinct advantages over other analytical procedures thus far employed for measuring PCA in that the 4-nitrophenacyl derivative of PCA can be readily prepared from deproteinized tissue homogenates and quantitated by HPLC within relatively short time intervals with good precision and specificity.

Original languageEnglish
Pages (from-to)135-140
Number of pages6
JournalAnalytical Biochemistry
Volume131
Issue number1
DOIs
Publication statusPublished - 1983

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Fingerprint Dive into the research topics of 'Analysis of 5-pyrrolidone-2-carboxylate ester by reverse phase high-performance liquid chromatography'. Together they form a unique fingerprint.

Cite this