Analysis of 5-pyrrolidone-2-carboxylate ester by reverse phase high-performance liquid chromatography

E. Bousquet; V. Guarcello; M. C. Morale; V. Rizza

doi:10.1016/0003-2697(83)90144-6

Analysis of 5-pyrrolidone-2-carboxylate ester by reverse phase high-performance liquid chromatography

E. Bousquet, V. Guarcello, M. C. Morale, V. Rizza

www.irccs.oasi.en.it

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Abstract

A simple quantitative method for estimating nanomole concentrations of 5-pyrrolidone-2-carboxylic acid (PCA) in tissue homogenates from mouse has been developed using reversephase HPLC. PCA was detected as the 4-nitrophenacyl ester which has an absorption maximum at 263 nm, a relatively high stability, and excellent chromatographic separation and detectability. This method offers distinct advantages over other analytical procedures thus far employed for measuring PCA in that the 4-nitrophenacyl derivative of PCA can be readily prepared from deproteinized tissue homogenates and quantitated by HPLC within relatively short time intervals with good precision and specificity.

Original language	English
Pages (from-to)	135-140
Number of pages	6
Journal	Analytical Biochemistry
Volume	131
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(83)90144-6
Publication status	Published - 1983

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/0003-2697(83)90144-6

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abstract = "A simple quantitative method for estimating nanomole concentrations of 5-pyrrolidone-2-carboxylic acid (PCA) in tissue homogenates from mouse has been developed using reversephase HPLC. PCA was detected as the 4-nitrophenacyl ester which has an absorption maximum at 263 nm, a relatively high stability, and excellent chromatographic separation and detectability. This method offers distinct advantages over other analytical procedures thus far employed for measuring PCA in that the 4-nitrophenacyl derivative of PCA can be readily prepared from deproteinized tissue homogenates and quantitated by HPLC within relatively short time intervals with good precision and specificity.",

author = "E. Bousquet and V. Guarcello and Morale, {M. C.} and V. Rizza",

year = "1983",

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AU - Rizza, V.

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AB - A simple quantitative method for estimating nanomole concentrations of 5-pyrrolidone-2-carboxylic acid (PCA) in tissue homogenates from mouse has been developed using reversephase HPLC. PCA was detected as the 4-nitrophenacyl ester which has an absorption maximum at 263 nm, a relatively high stability, and excellent chromatographic separation and detectability. This method offers distinct advantages over other analytical procedures thus far employed for measuring PCA in that the 4-nitrophenacyl derivative of PCA can be readily prepared from deproteinized tissue homogenates and quantitated by HPLC within relatively short time intervals with good precision and specificity.

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Analysis of 5-pyrrolidone-2-carboxylate ester by reverse phase high-performance liquid chromatography

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