Analysis of a polymerase chain reaction-amplified product of the DXS 164 locus in the dystrophin gene. Detection by capillary electrophoresis of restriction fragment length polymorphism

Domenico Del Principe, Maria Paola Iampieri, Daniela Germani, Adriana Menichelli, Giuseppe Novelli, Bruno Dallapiccola

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Abstract

Capillary electrophoresis (CE) was used to characterize restriction fragment length polymorphism (RFLP) in a polymerase chain reaction (PCR)-amplified product of a 740-base pairs DNA fragment from the DXS 164 locus of the dystrophin gene. The polymorphic alleles of 740 and 520/220 base pairs revealed by XmnI digestion were analysed from homozygous and heterozygous individuals by CE. Our studies show that extraction in phenol-chloroform may be useful in PCR-amplified product purification. Excellent separation was obtained in a short time. The data indicate that CE is suitable for genomic analysis such as carrier detection and prenatal diagnosis of X-linked recessive disorders after purification of PCR-amplified products.

Original languageEnglish
Pages (from-to)277-281
Number of pages5
JournalJournal of Chromatography A
Volume638
Issue number2
DOIs
Publication statusPublished - May 28 1993

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ASJC Scopus subject areas

  • Analytical Chemistry

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