Abstract
Capillary electrophoresis (CE) was used to characterize restriction fragment length polymorphism (RFLP) in a polymerase chain reaction (PCR)-amplified product of a 740-base pairs DNA fragment from the DXS 164 locus of the dystrophin gene. The polymorphic alleles of 740 and 520/220 base pairs revealed by XmnI digestion were analysed from homozygous and heterozygous individuals by CE. Our studies show that extraction in phenol-chloroform may be useful in PCR-amplified product purification. Excellent separation was obtained in a short time. The data indicate that CE is suitable for genomic analysis such as carrier detection and prenatal diagnosis of X-linked recessive disorders after purification of PCR-amplified products.
| Original language | English |
|---|---|
| Pages (from-to) | 277-281 |
| Number of pages | 5 |
| Journal | Journal of Chromatography A |
| Volume | 638 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - May 28 1993 |
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ASJC Scopus subject areas
- Analytical Chemistry
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Analysis of a polymerase chain reaction-amplified product of the DXS 164 locus in the dystrophin gene. Detection by capillary electrophoresis of restriction fragment length polymorphism. / Del Principe, Domenico; Paola Iampieri, Maria; Germani, Daniela; Menichelli, Adriana; Novelli, Giuseppe; Dallapiccola, Bruno.
In: Journal of Chromatography A, Vol. 638, No. 2, 28.05.1993, p. 277-281.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Analysis of a polymerase chain reaction-amplified product of the DXS 164 locus in the dystrophin gene. Detection by capillary electrophoresis of restriction fragment length polymorphism
AU - Del Principe, Domenico
AU - Paola Iampieri, Maria
AU - Germani, Daniela
AU - Menichelli, Adriana
AU - Novelli, Giuseppe
AU - Dallapiccola, Bruno
PY - 1993/5/28
Y1 - 1993/5/28
N2 - Capillary electrophoresis (CE) was used to characterize restriction fragment length polymorphism (RFLP) in a polymerase chain reaction (PCR)-amplified product of a 740-base pairs DNA fragment from the DXS 164 locus of the dystrophin gene. The polymorphic alleles of 740 and 520/220 base pairs revealed by XmnI digestion were analysed from homozygous and heterozygous individuals by CE. Our studies show that extraction in phenol-chloroform may be useful in PCR-amplified product purification. Excellent separation was obtained in a short time. The data indicate that CE is suitable for genomic analysis such as carrier detection and prenatal diagnosis of X-linked recessive disorders after purification of PCR-amplified products.
AB - Capillary electrophoresis (CE) was used to characterize restriction fragment length polymorphism (RFLP) in a polymerase chain reaction (PCR)-amplified product of a 740-base pairs DNA fragment from the DXS 164 locus of the dystrophin gene. The polymorphic alleles of 740 and 520/220 base pairs revealed by XmnI digestion were analysed from homozygous and heterozygous individuals by CE. Our studies show that extraction in phenol-chloroform may be useful in PCR-amplified product purification. Excellent separation was obtained in a short time. The data indicate that CE is suitable for genomic analysis such as carrier detection and prenatal diagnosis of X-linked recessive disorders after purification of PCR-amplified products.
UR - http://www.scopus.com/inward/record.url?scp=43949170546&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=43949170546&partnerID=8YFLogxK
U2 - 10.1016/0021-9673(93)83439-Y
DO - 10.1016/0021-9673(93)83439-Y
M3 - Article
VL - 638
SP - 277
EP - 281
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - 2
ER -