Analysis of a viral integration event in a CG-rich region at the 1p36 human chromosomal site

Massimo Romani, Ida Casciano, Flavia Querzola, Alessandro De Ambrosis, Marcello Siniscalco

Research output: Contribution to journalArticle

Abstract

The preinsertion site of an adenovirus-5/simian virus 40 recombinant construct (Ad5/SV40) has been cloned and sequenced. Our data suggest that viral integration has occurred in a genomic region which has been the target of multiple events of Alu element retropositions within a TAA minisatellite. Extensive homologies between the left viral end and the host cellular DNA were also observed. The compositional similarity between Adenoviridae and the region of viral integration is consistent with the observed insertion of exogenous DNA in isochores of similar composition [G. Bernardi, Annu. Rev. Genet. 23 (1989) 637-661].

Original languageEnglish
Pages (from-to)153-160
Number of pages8
JournalGene
Volume135
Issue number1-2
DOIs
Publication statusPublished - Dec 15 1993

Keywords

  • adenovirus type 5
  • HTF islands
  • illegitimate recombination
  • Recombinant DNA
  • repetitive Alu-like sequences
  • retroposons
  • SV40
  • viral-cellular junctions

ASJC Scopus subject areas

  • Genetics

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  • Cite this

    Romani, M., Casciano, I., Querzola, F., Ambrosis, A. D., & Siniscalco, M. (1993). Analysis of a viral integration event in a CG-rich region at the 1p36 human chromosomal site. Gene, 135(1-2), 153-160. https://doi.org/10.1016/0378-1119(93)90060-G