Analysis of CD3-associated molecules in CD4- 8- human T cell clones lacking the typical α/β T cell receptor

Cristina Bottino, Silvano Ferrini, Ermanno Ciccone, Ignazia Prigione, Daniela Pende, Maria Cristina Mingari, Lorenzo Moretta, Alessandro Moretta

Research output: Contribution to journalArticle

Abstract

Four clones were isolated from CD3+ 4- 8- normal peripheral blood T lymphocytes. They lacked expression of the typical α/β T cell receptor, as assessed by reactivity with the WT31 monoclonal antibody (mAb) recognizing a non-polymorphic structure of the α/β heterodimer. All the four clones lysed the natural killer-sensitive K562 target cells and released interleukin-2 following stimulation with phytohemagglutinin. After cell treatment with the cross-linking agent dithiobis-succinimidyl-propionate, molecules immunoprecipitated with an anti-CD3 mAb were represented by a major 45-kD and a minor 43-kD band. These molecules were different from typical α/β molecules and are likely to represent the molecular product of the human γ gene.

Original languageEnglish
Pages (from-to)125-129
Number of pages5
JournalLa Ricerca in Clinica e in Laboratorio
Volume17
Issue number2
DOIs
Publication statusPublished - Apr 1987

Keywords

  • γ T cell receptor
  • CD3-associated molecules
  • Immunoprecipitation
  • Interleukin-2
  • Monoclonal antibodies
  • T lymphocytes

ASJC Scopus subject areas

  • Clinical Biochemistry

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