Analysis of chromosomes 3, 7, X and the EGFR gene in uterine cervical cancer progression

R. Marzano; G. Corrado; R. Merola; C. Sbiroli; F. Guadagni; E. Vizza; F. Del Nonno; M. Carosi; M. Galati M; I. Sperduti; A. M. Cianciulli

doi:10.1016/j.ejca.2004.03.015

Analysis of chromosomes 3, 7, X and the EGFR gene in uterine cervical cancer progression

R. Marzano, G. Corrado, R. Merola, C. Sbiroli, F. Guadagni, E. Vizza, F. Del Nonno, M. Carosi, M. Galati M, I. Sperduti, A. M. Cianciulli

Research output: Contribution to journal › Article › peer-review

Abstract

The aim of this study was to investigate the possible role of genetic alterations in the genesis and progression of cervical carcinomas. We analysed the 3, 7, X aneusomy of chromosomes and the status of the epidermal growth factor receptor (EGFR) gene by fluorescence in situ hybridisation (FISH) analysis. Polysomy of chromosomes 3 and X defined the transition from high-grade squamous intraepithelium lesions (HSIL) to cervical carcinoma. Chromosome 7 monosomy and polysomy did not show any statistical significant differences between the groups examined. When we compared the chromosomal aneusomies in all of the specimens using the Kruskal-Wallis test, significant differences (P=0.0001, P=0.0001 for chromosomes 3 and X, respectively) were observed. Using a ratio of the EGFR gene signals and chromosome 7 centromeric signals, no samples showed gene amplification. Our results demonstrate the importance of chromosomal 3 and X aneusomies in the development and progression from HSIL to cervical carcinoma, highlighting their usefulness as genetic markers for identifying SILs at high-risk of progression.

Original language	English
Pages (from-to)	1624-1629
Number of pages	6
Journal	European Journal of Cancer
Volume	40
Issue number	10
DOIs	https://doi.org/10.1016/j.ejca.2004.03.015
Publication status	Published - Jul 2004

Keywords

Cervical carcinoma
Genetic markers
Progression

ASJC Scopus subject areas

Cancer Research
Hematology
Oncology

Access to Document

10.1016/j.ejca.2004.03.015

Fingerprint Dive into the research topics of 'Analysis of chromosomes 3, 7, X and the EGFR gene in uterine cervical cancer progression'. Together they form a unique fingerprint.

Cite this

Analysis of chromosomes 3, 7, X and the EGFR gene in uterine cervical cancer progression. / Marzano, R.; Corrado, G.; Merola, R.; Sbiroli, C.; Guadagni, F.; Vizza, E.; Del Nonno, F.; Carosi, M.; Galati M, M.; Sperduti, I.; Cianciulli, A. M.

In: European Journal of Cancer, Vol. 40, No. 10, 07.2004, p. 1624-1629.

Research output: Contribution to journal › Article › peer-review

@article{aa3d09f0759941f083c9864e2e466e41,

title = "Analysis of chromosomes 3, 7, X and the EGFR gene in uterine cervical cancer progression",

abstract = "The aim of this study was to investigate the possible role of genetic alterations in the genesis and progression of cervical carcinomas. We analysed the 3, 7, X aneusomy of chromosomes and the status of the epidermal growth factor receptor (EGFR) gene by fluorescence in situ hybridisation (FISH) analysis. Polysomy of chromosomes 3 and X defined the transition from high-grade squamous intraepithelium lesions (HSIL) to cervical carcinoma. Chromosome 7 monosomy and polysomy did not show any statistical significant differences between the groups examined. When we compared the chromosomal aneusomies in all of the specimens using the Kruskal-Wallis test, significant differences (P=0.0001, P=0.0001 for chromosomes 3 and X, respectively) were observed. Using a ratio of the EGFR gene signals and chromosome 7 centromeric signals, no samples showed gene amplification. Our results demonstrate the importance of chromosomal 3 and X aneusomies in the development and progression from HSIL to cervical carcinoma, highlighting their usefulness as genetic markers for identifying SILs at high-risk of progression.",

keywords = "Cervical carcinoma, Genetic markers, Progression",

author = "R. Marzano and G. Corrado and R. Merola and C. Sbiroli and F. Guadagni and E. Vizza and {Del Nonno}, F. and M. Carosi and {Galati M}, M. and I. Sperduti and Cianciulli, {A. M.}",

year = "2004",

month = jul,

doi = "10.1016/j.ejca.2004.03.015",

language = "English",

volume = "40",

pages = "1624--1629",

journal = "European Journal of Cancer",

issn = "0959-8049",

publisher = "Elsevier Ltd",

number = "10",

}

TY - JOUR

T1 - Analysis of chromosomes 3, 7, X and the EGFR gene in uterine cervical cancer progression

AU - Marzano, R.

AU - Corrado, G.

AU - Merola, R.

AU - Sbiroli, C.

AU - Guadagni, F.

AU - Vizza, E.

AU - Del Nonno, F.

AU - Carosi, M.

AU - Galati M, M.

AU - Sperduti, I.

AU - Cianciulli, A. M.

PY - 2004/7

Y1 - 2004/7

N2 - The aim of this study was to investigate the possible role of genetic alterations in the genesis and progression of cervical carcinomas. We analysed the 3, 7, X aneusomy of chromosomes and the status of the epidermal growth factor receptor (EGFR) gene by fluorescence in situ hybridisation (FISH) analysis. Polysomy of chromosomes 3 and X defined the transition from high-grade squamous intraepithelium lesions (HSIL) to cervical carcinoma. Chromosome 7 monosomy and polysomy did not show any statistical significant differences between the groups examined. When we compared the chromosomal aneusomies in all of the specimens using the Kruskal-Wallis test, significant differences (P=0.0001, P=0.0001 for chromosomes 3 and X, respectively) were observed. Using a ratio of the EGFR gene signals and chromosome 7 centromeric signals, no samples showed gene amplification. Our results demonstrate the importance of chromosomal 3 and X aneusomies in the development and progression from HSIL to cervical carcinoma, highlighting their usefulness as genetic markers for identifying SILs at high-risk of progression.

AB - The aim of this study was to investigate the possible role of genetic alterations in the genesis and progression of cervical carcinomas. We analysed the 3, 7, X aneusomy of chromosomes and the status of the epidermal growth factor receptor (EGFR) gene by fluorescence in situ hybridisation (FISH) analysis. Polysomy of chromosomes 3 and X defined the transition from high-grade squamous intraepithelium lesions (HSIL) to cervical carcinoma. Chromosome 7 monosomy and polysomy did not show any statistical significant differences between the groups examined. When we compared the chromosomal aneusomies in all of the specimens using the Kruskal-Wallis test, significant differences (P=0.0001, P=0.0001 for chromosomes 3 and X, respectively) were observed. Using a ratio of the EGFR gene signals and chromosome 7 centromeric signals, no samples showed gene amplification. Our results demonstrate the importance of chromosomal 3 and X aneusomies in the development and progression from HSIL to cervical carcinoma, highlighting their usefulness as genetic markers for identifying SILs at high-risk of progression.

KW - Cervical carcinoma

KW - Genetic markers

KW - Progression

UR - http://www.scopus.com/inward/record.url?scp=2942514601&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2942514601&partnerID=8YFLogxK

U2 - 10.1016/j.ejca.2004.03.015

DO - 10.1016/j.ejca.2004.03.015

M3 - Article

C2 - 15196550

AN - SCOPUS:2942514601

VL - 40

SP - 1624

EP - 1629

JO - European Journal of Cancer

JF - European Journal of Cancer

SN - 0959-8049

IS - 10

ER -