Analysis of coding and non-coding transcriptome of peripheral B cells reveals an altered interferon response factor (IRF)-1 pathway in multiple sclerosis patients

Viviana Annibali, Renato Umeton, Antonia Palermo, Martina Severa, Marilena Paola Etna, Simona Giglio, Silvia Romano, Michela Ferraldeschi, Maria Chiara Buscarinu, Andrea Vecchione, Anita Annese, Claudia Policano, Rosella Mechelli, Raffaella Pizzolato Umeton, Arianna Fornasiero, Daniela Francesca Angelini, Gisella Guerrera, Luca Battistini, Eliana Marina Coccia, Marco SalvettiGiovanni Ristori

Research output: Contribution to journalArticle

Abstract

Several evidences emphasize B-cell pathogenic roles in multiple sclerosis (MS). We performed transcriptome analyses on peripheral B cells from therapy-free patients and age/sex-matched controls. Down-regulation of two transcripts (interferon response factor 1–IRF1, and C-X-C motif chemokine 10–CXCL10), belonging to the same pathway, was validated by RT-PCR in 26 patients and 21 controls. IRF1 and CXCL10 transcripts share potential seeding sequences for hsa-miR-424, that resulted up-regulated in MS patients. We confirmed this interaction and its functional effect by transfection experiments. Consistent findings indicate down-regulation of IRF1/CXCL10 axis, that may plausibly contribute to a pro-survival status of B cells in MS.

Original languageEnglish
Pages (from-to)165-171
Number of pages7
JournalJournal of Neuroimmunology
Volume324
DOIs
Publication statusPublished - Nov 15 2018

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Keywords

  • B cells
  • CXCL10 axis
  • Hsa-miR-424
  • IRF-1
  • Multiple Sclerosis
  • Transcriptome analysis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

Cite this

Annibali, V., Umeton, R., Palermo, A., Severa, M., Etna, M. P., Giglio, S., Romano, S., Ferraldeschi, M., Buscarinu, M. C., Vecchione, A., Annese, A., Policano, C., Mechelli, R., Pizzolato Umeton, R., Fornasiero, A., Angelini, D. F., Guerrera, G., Battistini, L., Coccia, E. M., ... Ristori, G. (2018). Analysis of coding and non-coding transcriptome of peripheral B cells reveals an altered interferon response factor (IRF)-1 pathway in multiple sclerosis patients. Journal of Neuroimmunology, 324, 165-171. https://doi.org/10.1016/j.jneuroim.2018.09.005