Analysis of corticosteroids by high performance liquid chromatography- electrospray mass spectrometry

Alessia Panusa, Monica Ottaviani, Mauro Picardo, Emanuela Camera, Luigi Gagliardi, Paola Chimenti, Arianna Granese, Domenica Tonelli

Research output: Contribution to journalArticlepeer-review

Abstract

A high performance liquid chromatography electrospray mass spectrometry (HPLC-ESI-MS) method, for the detection of corticosteroids in cosmetics has been developed. A water-acetonitrile linear gradient on a C-18 reversed-phase column was found to be suitable in separating triamcinolone and its main derivatives, which greatly differ in lipophilicity. Detection was performed in negative electrospray ionisation mode. Good correlation between peaks areas and solutions concentration was found in the range 0.05-10.0 μg ml-1 and the detection limits resulted in the range of 20-45 pg injected. The method was successfully applied to the analysis of real samples of shampoo.

Original languageEnglish
Pages (from-to)719-723
Number of pages5
JournalAnalyst
Volume129
Issue number8
DOIs
Publication statusPublished - Aug 2004

ASJC Scopus subject areas

  • Analytical Chemistry

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