Analysis of differential miRNA expression in primary tumor and stroma of colorectal cancer patients

Giuseppina Della Vittoria Scarpati; Enrica Calura; Mariacristina Di Marino; Chiara Romualdi; Luca Beltrame; Umberto Malapelle; Giancarlo Troncone; Alfonso De Stefano; Stefano Pepe; Sabino De Placido; Maurizio D'Incalci; Sergio Marchini; Chiara Carlomagno

doi:10.1155/2014/840921

Analysis of differential miRNA expression in primary tumor and stroma of colorectal cancer patients

Giuseppina Della Vittoria Scarpati, Enrica Calura, Mariacristina Di Marino, Chiara Romualdi, Luca Beltrame, Umberto Malapelle, Giancarlo Troncone, Alfonso De Stefano, Stefano Pepe, Sabino De Placido, Maurizio D'Incalci, Sergio Marchini, Chiara Carlomagno

Istituto di Ricerche Farmacologiche "Mario Negri"

Research output: Contribution to journal › Article › peer-review

Abstract

Microarray technology was used to profile miRNA expression in primary tumor and stromal tissue from paraffin embedded material of 51 patients with colorectal cancer. 26 miRNAs resulted differentially expressed with at least 2-fold change in tumor tissue with respect to stroma (16 more expressed in the tumor and 10 more expressed in the stroma). 10/26 were confirmed as differentially expressed at qRTPCR: miR-200c-3p, miR-141-3p, miR-200b-3p, miR-200a-3p, miR-1246, miR-92a-3p, miR-194-5p, miR-192-5p, miR-3651-5p, and miR-574-3p. No significant association was found between miRNA expressions and stage at diagnosis, site of primary tumor, first site of metastasis, progression-free, or overall survival.

Original language	English
Article number	840921
Journal	BioMed Research International
Volume	2014
DOIs	https://doi.org/10.1155/2014/840921
Publication status	Published - 2014

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)
Medicine(all)

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Della Vittoria Scarpati, G., Calura, E., Di Marino, M., Romualdi, C., Beltrame, L., Malapelle, U., Troncone, G., De Stefano, A., Pepe, S., De Placido, S., D'Incalci, M., Marchini, S., & Carlomagno, C. (2014). Analysis of differential miRNA expression in primary tumor and stroma of colorectal cancer patients. BioMed Research International, 2014, [840921]. https://doi.org/10.1155/2014/840921

Analysis of differential miRNA expression in primary tumor and stroma of colorectal cancer patients. / Della Vittoria Scarpati, Giuseppina; Calura, Enrica; Di Marino, Mariacristina; Romualdi, Chiara; Beltrame, Luca; Malapelle, Umberto; Troncone, Giancarlo; De Stefano, Alfonso; Pepe, Stefano; De Placido, Sabino; D'Incalci, Maurizio ; Marchini, Sergio; Carlomagno, Chiara.

In: BioMed Research International, Vol. 2014, 840921, 2014.

Research output: Contribution to journal › Article › peer-review

Della Vittoria Scarpati, G, Calura, E, Di Marino, M, Romualdi, C, Beltrame, L, Malapelle, U, Troncone, G, De Stefano, A, Pepe, S, De Placido, S, D'Incalci, M , Marchini, S & Carlomagno, C 2014, 'Analysis of differential miRNA expression in primary tumor and stroma of colorectal cancer patients', BioMed Research International, vol. 2014, 840921. https://doi.org/10.1155/2014/840921

Della Vittoria Scarpati, Giuseppina ; Calura, Enrica ; Di Marino, Mariacristina ; Romualdi, Chiara ; Beltrame, Luca ; Malapelle, Umberto ; Troncone, Giancarlo ; De Stefano, Alfonso ; Pepe, Stefano ; De Placido, Sabino ; D'Incalci, Maurizio ; Marchini, Sergio ; Carlomagno, Chiara. / Analysis of differential miRNA expression in primary tumor and stroma of colorectal cancer patients. In: BioMed Research International. 2014 ; Vol. 2014.

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title = "Analysis of differential miRNA expression in primary tumor and stroma of colorectal cancer patients",

abstract = "Microarray technology was used to profile miRNA expression in primary tumor and stromal tissue from paraffin embedded material of 51 patients with colorectal cancer. 26 miRNAs resulted differentially expressed with at least 2-fold change in tumor tissue with respect to stroma (16 more expressed in the tumor and 10 more expressed in the stroma). 10/26 were confirmed as differentially expressed at qRTPCR: miR-200c-3p, miR-141-3p, miR-200b-3p, miR-200a-3p, miR-1246, miR-92a-3p, miR-194-5p, miR-192-5p, miR-3651-5p, and miR-574-3p. No significant association was found between miRNA expressions and stage at diagnosis, site of primary tumor, first site of metastasis, progression-free, or overall survival.",

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AU - Beltrame, Luca

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AU - Pepe, Stefano

AU - De Placido, Sabino

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AU - Marchini, Sergio

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AB - Microarray technology was used to profile miRNA expression in primary tumor and stromal tissue from paraffin embedded material of 51 patients with colorectal cancer. 26 miRNAs resulted differentially expressed with at least 2-fold change in tumor tissue with respect to stroma (16 more expressed in the tumor and 10 more expressed in the stroma). 10/26 were confirmed as differentially expressed at qRTPCR: miR-200c-3p, miR-141-3p, miR-200b-3p, miR-200a-3p, miR-1246, miR-92a-3p, miR-194-5p, miR-192-5p, miR-3651-5p, and miR-574-3p. No significant association was found between miRNA expressions and stage at diagnosis, site of primary tumor, first site of metastasis, progression-free, or overall survival.

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