Analysis of DNA microarrays by non-destructive fluorescent staining using SYBR® green II

C. Battaglia, G. Salani, C. Consolandi, L. Rossi Bernardi, Gianluca De Bellis

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

A simple, non-destructive procedure is described to determine the quality of DNA arrays before they are used. It consists of a preliminary staining step of the DNA microarray by using SYBR® green II, a fluorophore with specific affinity for ssDNA, followed by a laser scan analysis. The surface quality, integrity and homogeneity of each DNA spot of the array can thus be assessed. After this preliminary control, which may avoid further analytical steps that lead to the waste of precious biological samples, a fully reversible staining procedure is performed that produces an array ready for subsequent use.

Original languageEnglish
Pages (from-to)78-81
Number of pages4
JournalBioTechniques
Volume29
Issue number1
Publication statusPublished - 2000

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Microarrays
Oligonucleotide Array Sequence Analysis
Staining and Labeling
DNA
Fluorophores
Surface properties
Lasers
SYBR Green II

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Battaglia, C., Salani, G., Consolandi, C., Rossi Bernardi, L., & De Bellis, G. (2000). Analysis of DNA microarrays by non-destructive fluorescent staining using SYBR® green II. BioTechniques, 29(1), 78-81.

Analysis of DNA microarrays by non-destructive fluorescent staining using SYBR® green II. / Battaglia, C.; Salani, G.; Consolandi, C.; Rossi Bernardi, L.; De Bellis, Gianluca.

In: BioTechniques, Vol. 29, No. 1, 2000, p. 78-81.

Research output: Contribution to journalArticle

Battaglia, C, Salani, G, Consolandi, C, Rossi Bernardi, L & De Bellis, G 2000, 'Analysis of DNA microarrays by non-destructive fluorescent staining using SYBR® green II', BioTechniques, vol. 29, no. 1, pp. 78-81.
Battaglia, C. ; Salani, G. ; Consolandi, C. ; Rossi Bernardi, L. ; De Bellis, Gianluca. / Analysis of DNA microarrays by non-destructive fluorescent staining using SYBR® green II. In: BioTechniques. 2000 ; Vol. 29, No. 1. pp. 78-81.
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