Analysis of dominant copy number mutants of the plasmid pMB1

L. Castagnoli, R. M. Lacatena, G. Cesareni

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We characterize two dominant copy number mutants of a derivative of plasmid pMB1. One of the two mutations maps in the-35 region of the primer promoter and results in increased promoter activity. The analysis of the secondary structure in the proximity of the mutant sequence suggests a possible mechanism which could be the basis of the promoter-up phenotype. By comparing the properties of the mutant and the wild type plasmid in an in vitro system, we confirm that the primer and not its coding sequence is the target of RNA I inhibition. The second mutation affects the sequence of the primer so that it is less sensitive to inhibition by RNA I. We propose that this mutation stabilizes a secondary structure necessary for primer formation.

Original languageEnglish
Pages (from-to)5353-5367
Number of pages15
JournalNucleic Acids Research
Volume13
Issue number14
DOIs
Publication statusPublished - Jul 25 1985

Fingerprint

RNA
Promoter
Mutant
Mutation
Plasmids
Secondary Structure
Derivatives
Genetic Promoter Regions
Phenotype
Proximity
Coding
Derivative
Target
Necessary
RNA I

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Analysis of dominant copy number mutants of the plasmid pMB1. / Castagnoli, L.; Lacatena, R. M.; Cesareni, G.

In: Nucleic Acids Research, Vol. 13, No. 14, 25.07.1985, p. 5353-5367.

Research output: Contribution to journalArticle

Castagnoli, L. ; Lacatena, R. M. ; Cesareni, G. / Analysis of dominant copy number mutants of the plasmid pMB1. In: Nucleic Acids Research. 1985 ; Vol. 13, No. 14. pp. 5353-5367.
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