Analysis of fibronectin expression during human muscle differentiation.

G. Meola, E. Scarpini, M. Velicogna, A. Mottura, P. L. Baron, S. Beretta, G. Scarlato

Research output: Contribution to journalArticle

Abstract

Fibronectin expression during human muscle differentiation was investigated by determining its distribution in foetal, normal adult and dystrophic muscle and in foetal, normal adult and dystrophic muscle cultures during myogenesis. Muscle sections and muscle cultures were studied by indirect immunofluorescence staining using polyclonal and monoclonal anti-human antibodies. Mass and clonal muscle cultures were prepared from foetal, adult and dystrophic muscle tissue. Immunofluorescence staining detected fibronectin on the epimysium, perimysium and endomysium of transverse sections of normal adult muscle, while sarcoplasm was devoid of this glycoprotein. In foetal muscle, some fibers showed a prominent ring of fibronectin. In mass and clonal cultures, myoblasts were found to synthesize and accumulate fibronectin while myotubes did not. No difference in fibronectin distribution was observed between Duchenne Muscular Dystrophy (DMD) and control myotubes. An enzyme-linked immunoassay (ELISA), performed on homogenated muscle, sonicated fibroblasts and muscle cells, showed a high fibronectin level in fibroblasts when compared with the other samples tested.

Original languageEnglish
Pages (from-to)153-163
Number of pages11
JournalBasic and Applied Histochemistry
Volume30
Issue number2
Publication statusPublished - 1986

ASJC Scopus subject areas

  • Anatomy

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    Meola, G., Scarpini, E., Velicogna, M., Mottura, A., Baron, P. L., Beretta, S., & Scarlato, G. (1986). Analysis of fibronectin expression during human muscle differentiation. Basic and Applied Histochemistry, 30(2), 153-163.