Analysis of G(-174)C IL-6 polymorphism and plasma concentrations of inflammatory markers in patients with type 2 diabetes and peripheral arterial disease

M. Libra, S. S. Signorelli, Y. Bevelacqua, P. M. Navolanic, V. Bevelacqua, J. Polesel, R. Talamini, F. Stivala, M. C. Mazzarino, G. Malaponte

Research output: Contribution to journalArticle

Abstract

Aims: To determine whether the G(-174)C interleukin 6 (IL-6) polymorphism influences the development of peripheral arterial disease (PAD) in individuals with type 2 diabetes. This was investigated by comparing the distribution of G(-174)C genotypes between patients with type 2 diabetes and PAD (PAD +) and those with type 2 diabetes but without PAD (PAD-). Plasma concentrations of IL-6, fibrinogen, C reactive protein (CRP), and vascular endothelial growth factor (VEGF) were also compared in PAD+ and PAD- patients. Methods: Blood samples were collected from 146 PAD+ and 144 PAD- patients. SfaNI was used to determine the G(-174)C genotype. Plasma concentrations of IL-6, fibrinogen, CRP, and VEGF were measured by an enzyme linked immunosorbent assay. Results: The GG genotype was more common in PAD+ patients than in PAD- patients. PAD+ patients also had increased mean plasma concentrations of IL-6, fibrinogen, CRP, and VEGF compared with PAD- patients. Mean plasma concentrations of IL-6, fibrinogen, and CRP in both PAD+ and PAD - patients were higher in those with the GG genotype than in those with the GC or CC genotypes. In contrast, mean plasma concentrations of VEGF in PAD+ and PAD patients were not significantly different between those with different G(-174)C genotypes. Conclusions: These results support a model in which the GG genotype promotes PAD development among individuals with type 2 diabetes by inducing increased release of IL-6. Higher concentrations of IL-6 among those with the GG genotype is associated with increased plasma concentrations of fibrinogen and CRP.

Original languageEnglish
Pages (from-to)211-215
Number of pages5
JournalJournal of Clinical Pathology
Volume59
Issue number2
DOIs
Publication statusPublished - Feb 2006

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Fingerprint Dive into the research topics of 'Analysis of G(-174)C IL-6 polymorphism and plasma concentrations of inflammatory markers in patients with type 2 diabetes and peripheral arterial disease'. Together they form a unique fingerprint.

  • Cite this

    Libra, M., Signorelli, S. S., Bevelacqua, Y., Navolanic, P. M., Bevelacqua, V., Polesel, J., Talamini, R., Stivala, F., Mazzarino, M. C., & Malaponte, G. (2006). Analysis of G(-174)C IL-6 polymorphism and plasma concentrations of inflammatory markers in patients with type 2 diabetes and peripheral arterial disease. Journal of Clinical Pathology, 59(2), 211-215. https://doi.org/10.1136/jcp.2004.025452