Analysis of gene expression on anodic porous alumina microarrays.

Claudio Nicolini, Manjul Singh, Rosanna Spera, Lamberto Felli

Research output: Contribution to journalArticle

Abstract

This paper investigates the application of anodic porous alumina as an advancement on chip laboratory for gene expressions. The surface was prepared by a suitable electrolytic process to obtain a regular distribution of deep micrometric holes and printed bypen robot tips under standard conditions. The gene expression within the Nucleic Acid Programmable Protein Array (NAPPA) is realized in a confined environment of 16 spots, containing circular DNA plasmids expressed using rabbit reticulocyte lysate. Authors demonstrated the usefulness of APA in withholding the protein expression by detecting with a CCD microscope the photoluminescence signal emitted from the complex secondary antibody anchored to Cy3 and confined in the pores. Friction experiments proved the mechanical resistance under external stresses by the robot tip pens printing. So far, no attempts have been made to directly compare APA with any other surface/substrate; the rationale for pursuing APA as a potential surface coating is that it provides advantages over the simple functionalization of a glass slide, overcoming concerns about printing and its ability to generate viable arrays.

Original languageEnglish
Pages (from-to)332-337
Number of pages6
JournalBioengineered
Volume4
Issue number5
Publication statusPublished - Sep 2013

ASJC Scopus subject areas

  • Bioengineering
  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Medicine(all)

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  • Cite this

    Nicolini, C., Singh, M., Spera, R., & Felli, L. (2013). Analysis of gene expression on anodic porous alumina microarrays. Bioengineered, 4(5), 332-337.