Analysis of glycoproteins in human colon cancers, normal tissues and in human colon carcinoma cells reactive with monoclonal antibody NCL-19-9

Daniela Terracciano, Angela Mariano, Vincenzo Macchia, Angelina Di Carlo

Research output: Contribution to journalArticle

Abstract

The expression of sialyl Lewisa antigen, also known as Ca 19-9, in colon cancer, normal tissues and LS174T human colon carcinoma cells were studied. In colon adenocarcinoma and cell plasma membranes this antigen is expressed on various glycoproteins with different molecular weights ranging in size from over 200 kDa to about 100 kDa. In addition, there is very low expression in peritumoral tissues. In cytosol and culture medium this epitope is carried by a single complex-glycoprotein with a very high molecular weight resembling a mucin. In cells the rise in cAMP levels elevate the synthesis and release of the carbohydrate antigen 19-9; whereas the treatment with 1,9-dideoxyforskolin, a diterpene, which does not activate adenylate cyclase, has no effect on content of the antigen. These results suggest that cAMP is involved on the expression of glycoprotein-associated sialyl Lewisa antigen in LS174T cells.

Original languageEnglish
Pages (from-to)719-722
Number of pages4
JournalOncology Reports
Volume14
Issue number3
Publication statusPublished - Sep 2005

Keywords

  • Carbohydrate antigen 19-9
  • Colon cancer
  • Cyclic adenosine-monophosphate
  • Human colon carcinoma cell LS174T
  • Sialyl lewis antigen

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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