TY - JOUR
T1 - Analysis of glycoproteins in human colon cancers, normal tissues and in human colon carcinoma cells reactive with monoclonal antibody NCL-19-9
AU - Terracciano, Daniela
AU - Mariano, Angela
AU - Macchia, Vincenzo
AU - Di Carlo, Angelina
PY - 2005/9
Y1 - 2005/9
N2 - The expression of sialyl Lewisa antigen, also known as Ca 19-9, in colon cancer, normal tissues and LS174T human colon carcinoma cells were studied. In colon adenocarcinoma and cell plasma membranes this antigen is expressed on various glycoproteins with different molecular weights ranging in size from over 200 kDa to about 100 kDa. In addition, there is very low expression in peritumoral tissues. In cytosol and culture medium this epitope is carried by a single complex-glycoprotein with a very high molecular weight resembling a mucin. In cells the rise in cAMP levels elevate the synthesis and release of the carbohydrate antigen 19-9; whereas the treatment with 1,9-dideoxyforskolin, a diterpene, which does not activate adenylate cyclase, has no effect on content of the antigen. These results suggest that cAMP is involved on the expression of glycoprotein-associated sialyl Lewisa antigen in LS174T cells.
AB - The expression of sialyl Lewisa antigen, also known as Ca 19-9, in colon cancer, normal tissues and LS174T human colon carcinoma cells were studied. In colon adenocarcinoma and cell plasma membranes this antigen is expressed on various glycoproteins with different molecular weights ranging in size from over 200 kDa to about 100 kDa. In addition, there is very low expression in peritumoral tissues. In cytosol and culture medium this epitope is carried by a single complex-glycoprotein with a very high molecular weight resembling a mucin. In cells the rise in cAMP levels elevate the synthesis and release of the carbohydrate antigen 19-9; whereas the treatment with 1,9-dideoxyforskolin, a diterpene, which does not activate adenylate cyclase, has no effect on content of the antigen. These results suggest that cAMP is involved on the expression of glycoprotein-associated sialyl Lewisa antigen in LS174T cells.
KW - Carbohydrate antigen 19-9
KW - Colon cancer
KW - Cyclic adenosine-monophosphate
KW - Human colon carcinoma cell LS174T
KW - Sialyl lewis antigen
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M3 - Article
C2 - 16077981
AN - SCOPUS:24644492932
VL - 14
SP - 719
EP - 722
JO - Oncology Reports
JF - Oncology Reports
SN - 1021-335X
IS - 3
ER -