Analysis of heat-induced changes in protein expression of Stenotrophomonas maltophilia K279a reveals a role for GroEL in the host-temperature adaptation

Elena De Carolis, Brunella Posteraro, Ada Rita Florio, Bianca Colonna, Gianni Prosseda, Francesca Bugli, Silvia Renzetti Lorenzetti, Ersilia Fiscarelli, Rosanna Inzitari, Federica Iavarone, Massimo Castagnola, Giovanni Fadda, Maurizio Sanguinetti

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Stenotrophomonas maltophilia is a microorganism of environmental and clinical importance as well as a frequent airway colonizer of cystic fibrosis (CF) individuals. We combined 2-DE and MALDI-TOF MS to profile the protein expression in S. maltophilia K279a, a completely sequenced clinical isolate, grown at 37°C with respect to the strain grown at 26°C. Among the proteins up-regulated at 37°C, we identified GroEL, a molecular chaperone that mainly assist the folding and unfolding of proteins under both normal and stress conditions. A 2.4-kb groESL mRNA was detected independently by Northern blot analyses with a groES- and a groEL-specific probe, indicating that S. maltophilia groES and groEL form an operon. Primer extension analysis of S. maltophilia groESL done in Escherichia coli showed that 2 promoters, Pσ32 and Pσ70, were utilized under the heat-shock and normal condition, respectively, whereas S. maltophilia groEL was shown to act as a heat-shock gene at 37°C, 42°C, and, to a lesser extent, at 50°C by real-time RT-PCR analyses. Finally, immunoblot analyses revealed that S. maltophilia GroEL strongly reacted with sera from CF patients chronically infected by the microorganism, but did not with sera from CF patients with sporadic infection or uninfected.

Original languageEnglish
Pages (from-to)273-281
Number of pages9
JournalInternational journal of medical microbiology : IJMM
Volume301
Issue number4
DOIs
Publication statusPublished - Apr 2011

Fingerprint

Stenotrophomonas maltophilia
Hot Temperature
Temperature
Cystic Fibrosis
Proteins
Shock
Protein Unfolding
Molecular Chaperones
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Protein Folding
Operon
Serum
Northern Blotting
Real-Time Polymerase Chain Reaction
Escherichia coli
Messenger RNA
Infection
Genes

Keywords

  • GroEL
  • MALDI-TOF MS
  • S. maltophilia
  • Temperature-dependent expression

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases
  • Microbiology (medical)

Cite this

Analysis of heat-induced changes in protein expression of Stenotrophomonas maltophilia K279a reveals a role for GroEL in the host-temperature adaptation. / De Carolis, Elena; Posteraro, Brunella; Florio, Ada Rita; Colonna, Bianca; Prosseda, Gianni; Bugli, Francesca; Lorenzetti, Silvia Renzetti; Fiscarelli, Ersilia; Inzitari, Rosanna; Iavarone, Federica; Castagnola, Massimo; Fadda, Giovanni; Sanguinetti, Maurizio.

In: International journal of medical microbiology : IJMM, Vol. 301, No. 4, 04.2011, p. 273-281.

Research output: Contribution to journalArticle

De Carolis, E, Posteraro, B, Florio, AR, Colonna, B, Prosseda, G, Bugli, F, Lorenzetti, SR, Fiscarelli, E, Inzitari, R, Iavarone, F, Castagnola, M, Fadda, G & Sanguinetti, M 2011, 'Analysis of heat-induced changes in protein expression of Stenotrophomonas maltophilia K279a reveals a role for GroEL in the host-temperature adaptation', International journal of medical microbiology : IJMM, vol. 301, no. 4, pp. 273-281. https://doi.org/10.1016/j.ijmm.2010.10.001
De Carolis, Elena ; Posteraro, Brunella ; Florio, Ada Rita ; Colonna, Bianca ; Prosseda, Gianni ; Bugli, Francesca ; Lorenzetti, Silvia Renzetti ; Fiscarelli, Ersilia ; Inzitari, Rosanna ; Iavarone, Federica ; Castagnola, Massimo ; Fadda, Giovanni ; Sanguinetti, Maurizio. / Analysis of heat-induced changes in protein expression of Stenotrophomonas maltophilia K279a reveals a role for GroEL in the host-temperature adaptation. In: International journal of medical microbiology : IJMM. 2011 ; Vol. 301, No. 4. pp. 273-281.
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