Analysis of HIV-1- and CMV-specific memory CD4 T-cell responses during primary and chronic infection

Alexandre Harari, G. Paolo Rizzardi, Kim Ellefsen, Donatella Ciuffreda, Patrick Champagne, Pierre Alexandre Bart, Daniel Kaufmann, Amalio Telenti, Roland Sahli, Giuseppe Tambussi, Laurent Kaiser, Adriano Lazzarin, Luc Perrin, Giuseppe Pantaleo

Research output: Contribution to journalArticle

Abstract

CD4 T-cell-specific memory antiviral responses to human immunodeficiency virus type 1 (HIV-1) and cytomegalovirus (CMV) were investigated in 16 patients with documented primary HIV-1 infection (4 of the 16 subjects also had primary CMV infection) and compared with those observed in patients with chronic HIV-1 and CMV coinfection. Virus-specific memory CD4 T cells were characterized on the basis of the expression of the chemokine receptor CCR7. HIV-1- and CMV-specific interferon-γ-secreting CD4 T cells were detected in patients with primary and chronic HIV-1 and CMV coinfection and were mostly contained in the cell population lacking expression of CCR7. The magnitude of the primary CMV-specific CD4 T-cell response was significantly greater than that of chronic CMV infection, whereas there were no differences between primary and chronic HIV-1-specific CD4 T-cell responses. A substantial proportion of CD4+CCR7- T cells were infected with HIV-1. These results advance the characterization of antiviral memory CD4 T-cell response and the delineation of the potential mechanisms that likely prevent the generation of a robust CD4 T-cell immune response during primary infection.

Original languageEnglish
Pages (from-to)1381-1387
Number of pages7
JournalBlood
Volume100
Issue number4
DOIs
Publication statusPublished - Aug 15 2002

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T-cells
Cytomegalovirus
Viruses
HIV-1
T-Lymphocytes
Data storage equipment
Infection
Cytomegalovirus Infections
Coinfection
Antiviral Agents
Chemokine Receptors
Virus Diseases
Interferons
Cells

ASJC Scopus subject areas

  • Hematology

Cite this

Harari, A., Paolo Rizzardi, G., Ellefsen, K., Ciuffreda, D., Champagne, P., Bart, P. A., ... Pantaleo, G. (2002). Analysis of HIV-1- and CMV-specific memory CD4 T-cell responses during primary and chronic infection. Blood, 100(4), 1381-1387. https://doi.org/10.1182/blood-2001-11-0080

Analysis of HIV-1- and CMV-specific memory CD4 T-cell responses during primary and chronic infection. / Harari, Alexandre; Paolo Rizzardi, G.; Ellefsen, Kim; Ciuffreda, Donatella; Champagne, Patrick; Bart, Pierre Alexandre; Kaufmann, Daniel; Telenti, Amalio; Sahli, Roland; Tambussi, Giuseppe; Kaiser, Laurent; Lazzarin, Adriano; Perrin, Luc; Pantaleo, Giuseppe.

In: Blood, Vol. 100, No. 4, 15.08.2002, p. 1381-1387.

Research output: Contribution to journalArticle

Harari, A, Paolo Rizzardi, G, Ellefsen, K, Ciuffreda, D, Champagne, P, Bart, PA, Kaufmann, D, Telenti, A, Sahli, R, Tambussi, G, Kaiser, L, Lazzarin, A, Perrin, L & Pantaleo, G 2002, 'Analysis of HIV-1- and CMV-specific memory CD4 T-cell responses during primary and chronic infection', Blood, vol. 100, no. 4, pp. 1381-1387. https://doi.org/10.1182/blood-2001-11-0080
Harari A, Paolo Rizzardi G, Ellefsen K, Ciuffreda D, Champagne P, Bart PA et al. Analysis of HIV-1- and CMV-specific memory CD4 T-cell responses during primary and chronic infection. Blood. 2002 Aug 15;100(4):1381-1387. https://doi.org/10.1182/blood-2001-11-0080
Harari, Alexandre ; Paolo Rizzardi, G. ; Ellefsen, Kim ; Ciuffreda, Donatella ; Champagne, Patrick ; Bart, Pierre Alexandre ; Kaufmann, Daniel ; Telenti, Amalio ; Sahli, Roland ; Tambussi, Giuseppe ; Kaiser, Laurent ; Lazzarin, Adriano ; Perrin, Luc ; Pantaleo, Giuseppe. / Analysis of HIV-1- and CMV-specific memory CD4 T-cell responses during primary and chronic infection. In: Blood. 2002 ; Vol. 100, No. 4. pp. 1381-1387.
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