Analysis of HIV-1 reverse transcriptase gene mutations in infected children treated with zidovudine

Paola Orlandi, Caterina Cancrini, Stefania Scaccia, Maria Luisa Romiti, Susanna Livadiotti, Guido Castelli Gattinara, Federica Angelini, Susan Cox, Paolo Rossi

Research output: Contribution to journalArticlepeer-review

Abstract

Prolonged treatment with antiretroviral agents directed against reverse transcription (RT) in patients with HIV-1 infection results in the emergence of virus variants with reduced sensitivity containing mutations in the HIV-1 RT gene. Development of zidovudine (ZDV)-related mutations was studied in a cohort of 24 vertically infected pediatric patients receiving ZDV therapy. Monthly clinical and immunologic evaluation was accompanied by direct sequencing of the HIV-1 RT gene every 4 months. A correlation was observed between the emergence of mutations and the duration of therapy. Mutation at codon 41 was found only in the presence of mutation at codon 215. The presence of the mutations Met41→Leu and Thr215→Tyr/Phe did not appear to be related to disease progression. These findings suggest that the mere presence of mutations in the HIV-1 RT gene alone during ZDV monotherapy is not a reliable prognostic marker in the absence of other clinical and virologic information.

Original languageEnglish
Pages (from-to)230-237
Number of pages8
JournalJournal of Acquired Immune Deficiency Syndromes and Human Retrovirology
Volume19
Issue number3
Publication statusPublished - Nov 1 1998

Keywords

  • Children Zidovudine
  • HIV-1 infection
  • Prognostic marker
  • RT mutations

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Virology

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