Analysis of HLA-class-I specific natural killer cell receptors expressed on T lymphocytes infiltrating non-small-cell lung cancer

Claudia Semino, Guido Ferlazzo, Giovanni Battista Ratto, Giovanni Melioli

Research output: Contribution to journalArticle

Abstract

HLA-class I-specific natural killer cell receptors (HNKR) have been described to significantly interfere with both specific and non-specific functional activities of T lymphocytes. Despite the clear evidences obtained in T cells derived from peripheral blood, little is known about the activity of HNKR expressed in tumor infiltrating lymphocytes. For this reason, we have studied T lymphocytes derived from advanced non small cell lung cancers (NSCLC). The population of T cells expressing the HNKR+ phenotype was rare both in NSCLC-associated lymphocytes and in the peripheral blood. The two populations were clearly oligoclonal, as shown by the analysis of T cell receptor repertoire. Interestingly, while HNKR+ T cells derived from the peripheral blood belonged to the CD45R0 phenotype, the large majority of HNKR+ T cells in TIL were CD45RA. Functionally, all HNKR+ T cells displayed a cytolytic activity against allogeneic NSCLC. Autologous NSCLC, tested in a single patient, was lysed efficiently by HNKR+ T cells, thus suggesting that at least in this model, the presence of HNKR did not significantly interfere with the functional capacity of effector cells.

Original languageEnglish
Pages (from-to)395-405
Number of pages11
JournalLung Cancer
Volume34
Issue number3
Publication statusPublished - 2001

Keywords

  • HLA class I natural killer cell receptors
  • Natural killer cells
  • Non-small-cell lung cancer
  • T lymphocytes
  • Tumor infiltrating lymphocytes

ASJC Scopus subject areas

  • Oncology

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