Analysis of HLA-DP allelic sequence polymorphism using the in vitro enzymatic DNA amplification of DP-α and DP-β loci

T. L. Bugawan, G. T. Horn, C. M. Long, E. Mickelson, J. A. Hansen, G. B. Ferrara, G. Angelini, H. A. Erlich

Research output: Contribution to journalArticle

Abstract

Allelic sequence variation of the HLA DP-α and DP-β genes has been analyzed in a panel of 34 DP-typed cell lines. The polymorphic second exon of these genes was specifically amplified in vitro by the polymerase chain reaction method, using the thermostable DNA polymerase of Thermus, aquaticus. The analysis of M13 clones containing the amplified DP-β sequences revealed a total of 14 allelic variants. In general, specific allelic DP-β sequences were associated with each of the defined DPw1-w6 types, with β allele subtypes revealed for the DPw2 and DPw4 specificities. An additional six DP-β alleles which did not correlate with any of the T cell-defined specificities (DP 'blanks') were also identified. Only the two previously characterized alleles of DP-α were detected. These observations suggest that the T cell-defined DP specificities are determined by polymorphic residues on the β-chain. The sequence polymorphisms in DP-β are clustered in a few specific regions, and can be detected using sequence-specific oligonucleotide probes and polymerase chain reaction amplified DNA in a rapid dot-blot format. This approach provides a simple and informative method of DP typing. The DP-β sequences derived from four DP-typed celiac disease patients were compared with the distribution of DP-β alleles in control individuals.

Original languageEnglish
Pages (from-to)4024-4030
Number of pages7
JournalJournal of Immunology
Volume141
Issue number11
Publication statusPublished - 1988

ASJC Scopus subject areas

  • Immunology

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    Bugawan, T. L., Horn, G. T., Long, C. M., Mickelson, E., Hansen, J. A., Ferrara, G. B., Angelini, G., & Erlich, H. A. (1988). Analysis of HLA-DP allelic sequence polymorphism using the in vitro enzymatic DNA amplification of DP-α and DP-β loci. Journal of Immunology, 141(11), 4024-4030.