Analysis of HLA-DR polymorphism by two-dimensional peptide mapping

G. Corte, G. Damiani, F. Calabi, M. Fabbi, A. Bargellesi

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Abstract

Two-dimensional peptide mapping was used to study the polymorphism of DR antigens, membrane glycoproteins composed of two chains, α and β, and encoded by the human major histocompatibility complex (MHC). Four DR antigens were purified by immunoabsorption from four human lymphoblastoid cell lines homozygous at the DR locus. After labeling with 125I, α and β chains were separated by polyacrylamide gel electrophoresis in sodium dodecyl sulfate and digested with pepsin. Comparison of the peptide maps showed a marked degree of polymorphism among β chains: only 43% of peptides were common to all four chains and 15-21% of the spots were unique to a given chain. By contrast, only a limited variability was observed among α chains. Homology was 75% for the four chains and the percentage of unique peptides was very low. DR7 did not possess even a single unique peptide. The limited variability among α chains and the lack of 'private' peptides in one of them points to the conclusion that the β chain is the unique carrier of the alloantigenic specificities. Higher homology within the known crossreactive groups was not observed, suggesting that the determinants responsible for crossreactivity are on different molecules. From a genetic point of view, because β chains show allele-associated polymorphism, they are likely to be MHC encoded, whereas the minor differences among α chains do not allow a similar conclusion. The available data point to an analogy between these DR antigens and the mouse I-E/C antigens.

Original languageEnglish
Pages (from-to)534-538
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume78
Issue number1 II
DOIs
Publication statusPublished - 1981

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Peptide Mapping
HLA-DR Antigens
Peptides
Major Histocompatibility Complex
Antigens
Pepsin A
Membrane Glycoproteins
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
Alleles
Cell Line

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Analysis of HLA-DR polymorphism by two-dimensional peptide mapping. / Corte, G.; Damiani, G.; Calabi, F.; Fabbi, M.; Bargellesi, A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 78, No. 1 II, 1981, p. 534-538.

Research output: Contribution to journalArticle

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