Abstract
Some genetic determinants of longevity might reside in those polymorphisms for the immune system genes that regulate immune responses. Many longevity association studies focused their attention on HLA (the human MHC) polymorphisms, but discordant results have been obtained. Sardinians are a relatively isolate population and represent a suitable population for association studies. Some HLA-DR and DQ alleles form very stable haplotypes with a strong linkage disequilibrium. In a previous study on Sardinian centenarians we have suggested that HLA-DRB1*15 allele might be marginally associated to longevity. HLA-DR,DQ haplotypes are in strong linkage disequilibrium and well conserved playing a role in the association to diseases. Hence, we have evaluated, by amplification refractory mutation system/polymerase chain reaction (ARMS-PCR) the HLADQA1 and HLA-DQB1 allele frequencies in 123 centenarians and 92 controls from Sardinia to assess whether the association to HLA-DRB1*15 allele may be due to the other genes involved in the HLA-DR,DQ haplotypes. The frequencies of HLA-DQA1,DQB1 haplotypes were not significantly modified in centenarians. Nevertheless by evaluating the frequency of DRB1*15 linked haplotypes, we observed a not significant increase in centenarians of HLA-DQA1*01,DQB1*05 and HLA-DQA1*01,DQB1*06 haplotypes. These data suggest that these haplotypes might have a role in determining life span expectancy and longevity.
| Original language | English |
|---|---|
| Pages (from-to) | 114-118 |
| Number of pages | 5 |
| Journal | Experimental Gerontology |
| Volume | 43 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 2008 |
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Keywords
- Centenarians
- HLA-DQA1
- HLA-DQB1
- Immune response
- Longevity
- Sardinia
ASJC Scopus subject areas
- Ageing
- Medicine(all)
Cite this
Analysis of HLA-DRB1,DQA1,DQB1 haplotypes in Sardinian centenarians. / Scola, Letizia; Lio, Domenico; Candore, Giuseppina; Forte, Giusi I.; Crivello, Antonio; Colonna-Romano, Giuseppina; Pes, Mario G.; Carru, Ciriaco; Ferrucci, Luigi; Deiana, Luca; Baggio, Giovannella; Franceschi, Claudio; Caruso, Calogero.
In: Experimental Gerontology, Vol. 43, No. 2, 02.2008, p. 114-118.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Analysis of HLA-DRB1,DQA1,DQB1 haplotypes in Sardinian centenarians
AU - Scola, Letizia
AU - Lio, Domenico
AU - Candore, Giuseppina
AU - Forte, Giusi I.
AU - Crivello, Antonio
AU - Colonna-Romano, Giuseppina
AU - Pes, Mario G.
AU - Carru, Ciriaco
AU - Ferrucci, Luigi
AU - Deiana, Luca
AU - Baggio, Giovannella
AU - Franceschi, Claudio
AU - Caruso, Calogero
PY - 2008/2
Y1 - 2008/2
N2 - Some genetic determinants of longevity might reside in those polymorphisms for the immune system genes that regulate immune responses. Many longevity association studies focused their attention on HLA (the human MHC) polymorphisms, but discordant results have been obtained. Sardinians are a relatively isolate population and represent a suitable population for association studies. Some HLA-DR and DQ alleles form very stable haplotypes with a strong linkage disequilibrium. In a previous study on Sardinian centenarians we have suggested that HLA-DRB1*15 allele might be marginally associated to longevity. HLA-DR,DQ haplotypes are in strong linkage disequilibrium and well conserved playing a role in the association to diseases. Hence, we have evaluated, by amplification refractory mutation system/polymerase chain reaction (ARMS-PCR) the HLADQA1 and HLA-DQB1 allele frequencies in 123 centenarians and 92 controls from Sardinia to assess whether the association to HLA-DRB1*15 allele may be due to the other genes involved in the HLA-DR,DQ haplotypes. The frequencies of HLA-DQA1,DQB1 haplotypes were not significantly modified in centenarians. Nevertheless by evaluating the frequency of DRB1*15 linked haplotypes, we observed a not significant increase in centenarians of HLA-DQA1*01,DQB1*05 and HLA-DQA1*01,DQB1*06 haplotypes. These data suggest that these haplotypes might have a role in determining life span expectancy and longevity.
AB - Some genetic determinants of longevity might reside in those polymorphisms for the immune system genes that regulate immune responses. Many longevity association studies focused their attention on HLA (the human MHC) polymorphisms, but discordant results have been obtained. Sardinians are a relatively isolate population and represent a suitable population for association studies. Some HLA-DR and DQ alleles form very stable haplotypes with a strong linkage disequilibrium. In a previous study on Sardinian centenarians we have suggested that HLA-DRB1*15 allele might be marginally associated to longevity. HLA-DR,DQ haplotypes are in strong linkage disequilibrium and well conserved playing a role in the association to diseases. Hence, we have evaluated, by amplification refractory mutation system/polymerase chain reaction (ARMS-PCR) the HLADQA1 and HLA-DQB1 allele frequencies in 123 centenarians and 92 controls from Sardinia to assess whether the association to HLA-DRB1*15 allele may be due to the other genes involved in the HLA-DR,DQ haplotypes. The frequencies of HLA-DQA1,DQB1 haplotypes were not significantly modified in centenarians. Nevertheless by evaluating the frequency of DRB1*15 linked haplotypes, we observed a not significant increase in centenarians of HLA-DQA1*01,DQB1*05 and HLA-DQA1*01,DQB1*06 haplotypes. These data suggest that these haplotypes might have a role in determining life span expectancy and longevity.
KW - Centenarians
KW - HLA-DQA1
KW - HLA-DQB1
KW - Immune response
KW - Longevity
KW - Sardinia
UR - http://www.scopus.com/inward/record.url?scp=38149114647&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=38149114647&partnerID=8YFLogxK
U2 - 10.1016/j.exger.2007.06.007
DO - 10.1016/j.exger.2007.06.007
M3 - Article
C2 - 17714903
AN - SCOPUS:38149114647
VL - 43
SP - 114
EP - 118
JO - Experimental Gerontology
JF - Experimental Gerontology
SN - 0531-5565
IS - 2
ER -