Analysis of HLA specificity of human monoclonal antibodies by cytofluorimetry and cell ELISA

M. P. Pistillo, P. L. Tazzari, O. Mazzoleni, A. Urlacher, M. Falco, M. Vitale, R. W. Karr, G. B. Ferrara

Research output: Contribution to journalArticle

Abstract

In this study we report on the characterization of cytotoxic human monoclonal antibodies (HmAb) detecting polymorphic HLA class II specificities using cytofluorimetric analysis in combination with micro cell ELISA. In both techniques, five anti-HLA HmAb were tested against HLA-transfected murine L cells as target cells and the bound antibody was detected, either by cytofluorimetry or by cell ELISA reader, after addition of fluoresceinated or peroxidase-conjugated anti-human IgG+IgM antibodies, respectively . The results demonstrate that HmAb directed against HLA-DR, -DQ and -DP molecules can be efficiently discriminated by cytofluorimetry and cell ELISA. which appear to be highly sensitive and perfectly comparable to the standard cytotoxicity assay.

Original languageEnglish
Pages (from-to)345-353
Number of pages9
JournalEuropean Journal of Immunogenetics
Volume18
Issue number5-6
Publication statusPublished - 1991

Fingerprint

Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
HLA-DQ Antigens
Antibodies
HLA-DR Antigens
Peroxidase
Immunoglobulin M

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Analysis of HLA specificity of human monoclonal antibodies by cytofluorimetry and cell ELISA. / Pistillo, M. P.; Tazzari, P. L.; Mazzoleni, O.; Urlacher, A.; Falco, M.; Vitale, M.; Karr, R. W.; Ferrara, G. B.

In: European Journal of Immunogenetics, Vol. 18, No. 5-6, 1991, p. 345-353.

Research output: Contribution to journalArticle

Pistillo, MP, Tazzari, PL, Mazzoleni, O, Urlacher, A, Falco, M, Vitale, M, Karr, RW & Ferrara, GB 1991, 'Analysis of HLA specificity of human monoclonal antibodies by cytofluorimetry and cell ELISA', European Journal of Immunogenetics, vol. 18, no. 5-6, pp. 345-353.
Pistillo, M. P. ; Tazzari, P. L. ; Mazzoleni, O. ; Urlacher, A. ; Falco, M. ; Vitale, M. ; Karr, R. W. ; Ferrara, G. B. / Analysis of HLA specificity of human monoclonal antibodies by cytofluorimetry and cell ELISA. In: European Journal of Immunogenetics. 1991 ; Vol. 18, No. 5-6. pp. 345-353.
@article{8524e97528d540069e07d27beb2b7d78,
title = "Analysis of HLA specificity of human monoclonal antibodies by cytofluorimetry and cell ELISA",
abstract = "In this study we report on the characterization of cytotoxic human monoclonal antibodies (HmAb) detecting polymorphic HLA class II specificities using cytofluorimetric analysis in combination with micro cell ELISA. In both techniques, five anti-HLA HmAb were tested against HLA-transfected murine L cells as target cells and the bound antibody was detected, either by cytofluorimetry or by cell ELISA reader, after addition of fluoresceinated or peroxidase-conjugated anti-human IgG+IgM antibodies, respectively . The results demonstrate that HmAb directed against HLA-DR, -DQ and -DP molecules can be efficiently discriminated by cytofluorimetry and cell ELISA. which appear to be highly sensitive and perfectly comparable to the standard cytotoxicity assay.",
author = "Pistillo, {M. P.} and Tazzari, {P. L.} and O. Mazzoleni and A. Urlacher and M. Falco and M. Vitale and Karr, {R. W.} and Ferrara, {G. B.}",
year = "1991",
language = "English",
volume = "18",
pages = "345--353",
journal = "European Journal of Immunogenetics",
issn = "0960-7420",
publisher = "Wiley-Blackwell",
number = "5-6",

}

TY - JOUR

T1 - Analysis of HLA specificity of human monoclonal antibodies by cytofluorimetry and cell ELISA

AU - Pistillo, M. P.

AU - Tazzari, P. L.

AU - Mazzoleni, O.

AU - Urlacher, A.

AU - Falco, M.

AU - Vitale, M.

AU - Karr, R. W.

AU - Ferrara, G. B.

PY - 1991

Y1 - 1991

N2 - In this study we report on the characterization of cytotoxic human monoclonal antibodies (HmAb) detecting polymorphic HLA class II specificities using cytofluorimetric analysis in combination with micro cell ELISA. In both techniques, five anti-HLA HmAb were tested against HLA-transfected murine L cells as target cells and the bound antibody was detected, either by cytofluorimetry or by cell ELISA reader, after addition of fluoresceinated or peroxidase-conjugated anti-human IgG+IgM antibodies, respectively . The results demonstrate that HmAb directed against HLA-DR, -DQ and -DP molecules can be efficiently discriminated by cytofluorimetry and cell ELISA. which appear to be highly sensitive and perfectly comparable to the standard cytotoxicity assay.

AB - In this study we report on the characterization of cytotoxic human monoclonal antibodies (HmAb) detecting polymorphic HLA class II specificities using cytofluorimetric analysis in combination with micro cell ELISA. In both techniques, five anti-HLA HmAb were tested against HLA-transfected murine L cells as target cells and the bound antibody was detected, either by cytofluorimetry or by cell ELISA reader, after addition of fluoresceinated or peroxidase-conjugated anti-human IgG+IgM antibodies, respectively . The results demonstrate that HmAb directed against HLA-DR, -DQ and -DP molecules can be efficiently discriminated by cytofluorimetry and cell ELISA. which appear to be highly sensitive and perfectly comparable to the standard cytotoxicity assay.

UR - http://www.scopus.com/inward/record.url?scp=0026045032&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026045032&partnerID=8YFLogxK

M3 - Article

C2 - 1772879

AN - SCOPUS:0026045032

VL - 18

SP - 345

EP - 353

JO - European Journal of Immunogenetics

JF - European Journal of Immunogenetics

SN - 0960-7420

IS - 5-6

ER -