Analysis of HLA specificity of human monoclonal antibodies by cytofluorimetry and cell ELISA

M. P. Pistillo, P. L. Tazzari, O. Mazzoleni, A. Urlacher, M. Falco, M. Vitale, R. W. Karr, G. B. Ferrara

Research output: Contribution to journalArticle

Abstract

In this study we report on the characterization of cytotoxic human monoclonal antibodies (HmAb) detecting polymorphic HLA class II specificities using cytofluorimetric analysis in combination with micro cell ELISA. In both techniques, five anti-HLA HmAb were tested against HLA-transfected murine L cells as target cells and the bound antibody was detected, either by cytofluorimetry or by cell ELISA reader, after addition of fluoresceinated or peroxidase-conjugated anti-human IgG+IgM antibodies, respectively . The results demonstrate that HmAb directed against HLA-DR, -DQ and -DP molecules can be efficiently discriminated by cytofluorimetry and cell ELISA. which appear to be highly sensitive and perfectly comparable to the standard cytotoxicity assay.

Original languageEnglish
Pages (from-to)345-353
Number of pages9
JournalEuropean Journal of Immunogenetics
Volume18
Issue number5-6
Publication statusPublished - 1991

ASJC Scopus subject areas

  • Immunology
  • Genetics

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