Analysis of MGMT promoter methylation status on intraoperative fresh tissue section from frameless neuronavigation needle biopsy: A preliminary study of ten patients

Corrado Iaccarino, Davide Nicoli, Carmine Gallo, Davide Nasi, Anna Pisanello, Gianni De Berti, Reza Ghadirpour, Norina Marcello, Franco Servadei

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background After a brain biopsy, the genetic analysis can fail because of insufficient material, extensive tumor necrosis, and formalin fixation under conditions that adversely affected the quality of the DNA or because the assay result was indeterminant. The freezing of fresh tumor tissue at surgery could greatly improve the success of DNA extraction and methyl guanine methyl transferase (MGMT) promoter methylation testing. The concentration of the DNA samples can also be improved from a withdrawal in an area with a high probability of neoplastic cells. Methods The present study reports the results of ten frameless image-guided intracranial needle biopsies from April 2008 until February 2009, among a total of 28 frameless neuronavigation brain biopsy performed from May 2007 to February 2009. The protocol sampling provided withdrawal specimens correlated with neuroimaging characteristics of the lesions. The molecular determination of MGMT promoter was assessed with the nested methylation-specific polymerase chain reaction on fresh or cryopreserved needle bioptic tissue. Results The genetic characterization was feasible in all the bioptic samples. The MGMT promoter was methylated in six patients, including a brain infection. The image-guided trajectory of the biopsy and the intraoperative frozen section increased the diagnostic yield. Conclusions To the best of the authors' knowledge, this is the first report with the MGMT promoter status analysis on needle bioptic fresh tissue. In the future, the availability of the molecular genetic characterization of a brain tumor before open surgery will provide important information for the optimal treatment.

Original languageEnglish
Pages (from-to)1189-1196
Number of pages8
JournalActa Neurochirurgica
Volume152
Issue number7
DOIs
Publication statusPublished - Jul 2010

Fingerprint

Neuronavigation
Guanine
Needle Biopsy
Transferases
Methylation
Needles
DNA
Brain
Image-Guided Biopsy
Biopsy
Frozen Sections
Neuroimaging
Brain Neoplasms
Formaldehyde
Freezing
Molecular Biology
Neoplasms
Necrosis
Polymerase Chain Reaction
Infection

Keywords

  • Biopsy
  • Frameless
  • MGMT
  • Molecular analysis
  • MSP
  • Neuronavigation

ASJC Scopus subject areas

  • Clinical Neurology
  • Surgery

Cite this

Analysis of MGMT promoter methylation status on intraoperative fresh tissue section from frameless neuronavigation needle biopsy : A preliminary study of ten patients. / Iaccarino, Corrado; Nicoli, Davide; Gallo, Carmine; Nasi, Davide; Pisanello, Anna; Berti, Gianni De; Ghadirpour, Reza; Marcello, Norina; Servadei, Franco.

In: Acta Neurochirurgica, Vol. 152, No. 7, 07.2010, p. 1189-1196.

Research output: Contribution to journalArticle

Iaccarino, Corrado ; Nicoli, Davide ; Gallo, Carmine ; Nasi, Davide ; Pisanello, Anna ; Berti, Gianni De ; Ghadirpour, Reza ; Marcello, Norina ; Servadei, Franco. / Analysis of MGMT promoter methylation status on intraoperative fresh tissue section from frameless neuronavigation needle biopsy : A preliminary study of ten patients. In: Acta Neurochirurgica. 2010 ; Vol. 152, No. 7. pp. 1189-1196.
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