Abstract
In this study we analyzed the expression of EB6 and GL183, which are part of P58 molecular family that represents the putative NK receptor for MHC class I molecules, in peripheral blood lymphocytes of 60 patients with HIV infection (20 asymptomatic HIV-seropositive individuals, 20 patients with constitutional symptoms, and 20 AIDS patients) and correlated it with the level of CD4+, CD56+ cells, and the NK cell activity in order to determine a possible relation with disease progression. The absolute number (but not the percentage) of CD56+, EB6+, and GL183+ cells was significantly reduced only in AIDS patients but not in the other AIDS-related clinical conditions. On the contrary, NK cell activity was reduced in all HIV-infected patients. In a 6-month follow-up, patients with constant clinical conditions and stable CD4+ cells level showed no significant difference, either in the percentage or absolute number of EB6+ and GL183+ cells. Interestingly, dual-color fluorescence indicates that GL183 and EB6 molecules (that in normal individuals are virtually absent on CD3- NK cells) are expressed in HIV-infected individuals not only in CD56+ cells but also in CD3+ cells. This may reflect a depletion of other T cell subsets or alternatively (less likely) a specific immune response. Our data indicate that the expression of EB6 and GL183 in T and NK cells from HIV-infected patients might be relevant in the course of the disease and for the disease-associated functional defect of NK cell activity.
| Original language | English |
|---|---|
| Pages (from-to) | 198-205 |
| Number of pages | 8 |
| Journal | Clinical Immunology and Immunopathology |
| Volume | 70 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1994 |
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ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
- Pathology and Forensic Medicine
Cite this
Analysis of natural killer (NK) cell subsets defined by the expression of two novel surface antigens (EB6 and GL183) in AIDS and AIDS-related conditions. / Cauda, R.; Goletti, D.; Lucia, M. B.; Tumbarello, M.; Rumi, C.; Orengo, A. M.; Moretta, A.
In: Clinical Immunology and Immunopathology, Vol. 70, No. 3, 1994, p. 198-205.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Analysis of natural killer (NK) cell subsets defined by the expression of two novel surface antigens (EB6 and GL183) in AIDS and AIDS-related conditions
AU - Cauda, R.
AU - Goletti, D.
AU - Lucia, M. B.
AU - Tumbarello, M.
AU - Rumi, C.
AU - Orengo, A. M.
AU - Moretta, A.
PY - 1994
Y1 - 1994
N2 - In this study we analyzed the expression of EB6 and GL183, which are part of P58 molecular family that represents the putative NK receptor for MHC class I molecules, in peripheral blood lymphocytes of 60 patients with HIV infection (20 asymptomatic HIV-seropositive individuals, 20 patients with constitutional symptoms, and 20 AIDS patients) and correlated it with the level of CD4+, CD56+ cells, and the NK cell activity in order to determine a possible relation with disease progression. The absolute number (but not the percentage) of CD56+, EB6+, and GL183+ cells was significantly reduced only in AIDS patients but not in the other AIDS-related clinical conditions. On the contrary, NK cell activity was reduced in all HIV-infected patients. In a 6-month follow-up, patients with constant clinical conditions and stable CD4+ cells level showed no significant difference, either in the percentage or absolute number of EB6+ and GL183+ cells. Interestingly, dual-color fluorescence indicates that GL183 and EB6 molecules (that in normal individuals are virtually absent on CD3- NK cells) are expressed in HIV-infected individuals not only in CD56+ cells but also in CD3+ cells. This may reflect a depletion of other T cell subsets or alternatively (less likely) a specific immune response. Our data indicate that the expression of EB6 and GL183 in T and NK cells from HIV-infected patients might be relevant in the course of the disease and for the disease-associated functional defect of NK cell activity.
AB - In this study we analyzed the expression of EB6 and GL183, which are part of P58 molecular family that represents the putative NK receptor for MHC class I molecules, in peripheral blood lymphocytes of 60 patients with HIV infection (20 asymptomatic HIV-seropositive individuals, 20 patients with constitutional symptoms, and 20 AIDS patients) and correlated it with the level of CD4+, CD56+ cells, and the NK cell activity in order to determine a possible relation with disease progression. The absolute number (but not the percentage) of CD56+, EB6+, and GL183+ cells was significantly reduced only in AIDS patients but not in the other AIDS-related clinical conditions. On the contrary, NK cell activity was reduced in all HIV-infected patients. In a 6-month follow-up, patients with constant clinical conditions and stable CD4+ cells level showed no significant difference, either in the percentage or absolute number of EB6+ and GL183+ cells. Interestingly, dual-color fluorescence indicates that GL183 and EB6 molecules (that in normal individuals are virtually absent on CD3- NK cells) are expressed in HIV-infected individuals not only in CD56+ cells but also in CD3+ cells. This may reflect a depletion of other T cell subsets or alternatively (less likely) a specific immune response. Our data indicate that the expression of EB6 and GL183 in T and NK cells from HIV-infected patients might be relevant in the course of the disease and for the disease-associated functional defect of NK cell activity.
UR - http://www.scopus.com/inward/record.url?scp=0028351235&partnerID=8YFLogxK
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U2 - 10.1006/clin.1994.1029
DO - 10.1006/clin.1994.1029
M3 - Article
VL - 70
SP - 198
EP - 205
JO - Clinical Immunology and Immunopathology
JF - Clinical Immunology and Immunopathology
SN - 0090-1229
IS - 3
ER -