Analysis of octamer-binding transcription factors Oct2 and Oct1 and their coactivator BOB.1/OBF.1 in Lymphomas

Ana Isabel Sáez, Maria Jesús Artiga, Margarita Sánchez-Beato, Lydia Sánchez-Verde, Juan Fernando García, Francisca Inmaculada Camacho, Renato Franco, Miguel A. Piris

Research output: Contribution to journalArticle

Abstract

Oct1 and Oct2 are transcription factors of the POU homeo-domain family that bind to the Ig gene octamer sites, regulating B-cell-specific genes. The function of these transcription factors is dependent on the activity of B-cell-restricted coactivators such as BOB.1/OBF.1. Independent studies of the expression of these proteins in non-Hodgkin's lymphoma have been restricted to single markers, and most lack data concerning immunohistochemical expression. Thus, we have investigated the expression of Oct1, Oct2, and BOB.1/OBF.1 in human reactive lymphoid tissue and in a series of 140 Hodgkin and non-Hodgkin's lymphomas. None of these proteins was found to be restricted to B cells, although only B cells expressed high levels of all three markers. Additionally, germinal center B cells showed stronger Oct2 and BOB.1/OBF.1 staining. Consequently, most B-cell lymphomas showed reactivity for all three antibodies. Oct2 expression was significantly higher in germinal center-derived lymphomas, although other B-cell lymphomas also displayed a high level of Oct2 expression Although T-cell lymphomas and Hodgkin's lymphomas expressed some of these proteins, they commonly exhibited less reactivity than B-cell lymphomas. Despite not being entirely cell-specific, the strong nuclear expression of Oct2 and BOB.1/OBF.1 by germinal center-derived lymphomas makes these antibodies a potentially useful tool in lymphoma diagnosis.

Original languageEnglish
Pages (from-to)211-220
Number of pages10
JournalModern Pathology
Volume15
Issue number3
DOIs
Publication statusPublished - 2002

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Keywords

  • BOB1
  • Lymphoma
  • Oct1
  • Oct2
  • Transcription factors

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Sáez, A. I., Artiga, M. J., Sánchez-Beato, M., Sánchez-Verde, L., García, J. F., Camacho, F. I., Franco, R., & Piris, M. A. (2002). Analysis of octamer-binding transcription factors Oct2 and Oct1 and their coactivator BOB.1/OBF.1 in Lymphomas. Modern Pathology, 15(3), 211-220. https://doi.org/10.1038/modpathol.3880518