Analysis of p53 and ras gene mutations in hepatocellular carcinoma in italy

Dario Conte, Antonino Neri, Nicola S. Fracchiolla, Lory S. Crocè, Lucia Lodi, Mirella Fraquelli, Flora Masuttit, Claudio Tiribellit

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To evaluate the frequency of p53 tumour suppressor gene and H- and K-ras oncogene mutations in both neoplastic and distant cirrhotic non-neoplastic tissue in Italian patients with hepatocellular carcinoma (HCC). Patients: Ten men and four women (mean age 62 ±7 years) with HCC associated with cirrhosis, who underwent resection. More than half the patients had a lesion over 3 cm in diameter and in 12 cases carcinoma was well differentiated. Methods: Exons 4 to 9 of p53 and codons 12, 13 and 61 of the H- and K-ras genes were analyzed by the polymerase chain reaction-single strand conformation polymorphism method in both neoplastic and non-adjacent cirrhotic tissue and in cell lines harbouring known mutations as positive controls. Results: No aberrant migrating fragments were observed in tumoral and cirrhotic non-neoplastic tissue. Conclusions: Our findings suggest that p53 mutation is a rare occurrence in HCC, at least in countries with a low incidence of this cancer. They also confirm that H- and K-ras mutations are infrequent in HCC and play a minimal role in hepatocarc inogenesis.

Original languageEnglish
Pages (from-to)1005-1008
Number of pages4
JournalEuropean Journal of Gastroenterology and Hepatology
Volume6
Issue number11
Publication statusPublished - 1994

Keywords

  • Cirrhosis
  • H- and K-ras oncogenes
  • Hepatocellular carcinoma
  • P53 tumour suppressor gene

ASJC Scopus subject areas

  • Gastroenterology
  • Hepatology

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