Analysis of protein ions in the range 3000-12000 Th under partial (no discharge) atmospheric pressure chemical ionization conditions using ion trap mass spectrometry

Simone Cristoni, Luigi Rossi Bernardi, Ida Biunno, Federico Guidugli

Research output: Contribution to journalArticlepeer-review

Abstract

A new approach, based on the use of atmospheric pressure chemical ionization ion trap mass spectrometry (APCI-ITMS), but without a corona discharge, was investigated for application to creating and monitoring protein ions. It must be emphasized that APCI is not usually used in protein analysis. In order to verify the applicability of the proposed method to the analysis of proteins, two standard proteins (horse cytochrome c and horse myoglobin) were analyzed. A mixture of the two proteins was also analyzed showing that this novel approach, based on the use of APCI, can be used in the analysis of protein mixtures.

Original languageEnglish
Pages (from-to)1153-1159
Number of pages7
JournalRapid Communications in Mass Spectrometry
Volume16
Issue number12
DOIs
Publication statusPublished - 2002

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

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