Abstract
A new approach, based on the use of atmospheric pressure chemical ionization ion trap mass spectrometry (APCI-ITMS), but without a corona discharge, was investigated for application to creating and monitoring protein ions. It must be emphasized that APCI is not usually used in protein analysis. In order to verify the applicability of the proposed method to the analysis of proteins, two standard proteins (horse cytochrome c and horse myoglobin) were analyzed. A mixture of the two proteins was also analyzed showing that this novel approach, based on the use of APCI, can be used in the analysis of protein mixtures.
| Original language | English |
|---|---|
| Pages (from-to) | 1153-1159 |
| Number of pages | 7 |
| Journal | Rapid Communications in Mass Spectrometry |
| Volume | 16 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 2002 |
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ASJC Scopus subject areas
- Analytical Chemistry
- Spectroscopy
Cite this
Analysis of protein ions in the range 3000-12000 Th under partial (no discharge) atmospheric pressure chemical ionization conditions using ion trap mass spectrometry. / Cristoni, Simone; Bernardi, Luigi Rossi; Biunno, Ida; Guidugli, Federico.
In: Rapid Communications in Mass Spectrometry, Vol. 16, No. 12, 2002, p. 1153-1159.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Analysis of protein ions in the range 3000-12000 Th under partial (no discharge) atmospheric pressure chemical ionization conditions using ion trap mass spectrometry
AU - Cristoni, Simone
AU - Bernardi, Luigi Rossi
AU - Biunno, Ida
AU - Guidugli, Federico
PY - 2002
Y1 - 2002
N2 - A new approach, based on the use of atmospheric pressure chemical ionization ion trap mass spectrometry (APCI-ITMS), but without a corona discharge, was investigated for application to creating and monitoring protein ions. It must be emphasized that APCI is not usually used in protein analysis. In order to verify the applicability of the proposed method to the analysis of proteins, two standard proteins (horse cytochrome c and horse myoglobin) were analyzed. A mixture of the two proteins was also analyzed showing that this novel approach, based on the use of APCI, can be used in the analysis of protein mixtures.
AB - A new approach, based on the use of atmospheric pressure chemical ionization ion trap mass spectrometry (APCI-ITMS), but without a corona discharge, was investigated for application to creating and monitoring protein ions. It must be emphasized that APCI is not usually used in protein analysis. In order to verify the applicability of the proposed method to the analysis of proteins, two standard proteins (horse cytochrome c and horse myoglobin) were analyzed. A mixture of the two proteins was also analyzed showing that this novel approach, based on the use of APCI, can be used in the analysis of protein mixtures.
UR - http://www.scopus.com/inward/record.url?scp=0035983402&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035983402&partnerID=8YFLogxK
U2 - 10.1002/rcm.693
DO - 10.1002/rcm.693
M3 - Article
C2 - 12112265
AN - SCOPUS:0035983402
VL - 16
SP - 1153
EP - 1159
JO - Rapid Communications in Mass Spectrometry
JF - Rapid Communications in Mass Spectrometry
SN - 0951-4198
IS - 12
ER -