Analysis of protein ions in the range 3000-12000 Th under partial (no discharge) atmospheric pressure chemical ionization conditions using ion trap mass spectrometry

Simone Cristoni, Luigi Rossi Bernardi, Ida Biunno, Federico Guidugli

Research output: Contribution to journalArticle

Abstract

A new approach, based on the use of atmospheric pressure chemical ionization ion trap mass spectrometry (APCI-ITMS), but without a corona discharge, was investigated for application to creating and monitoring protein ions. It must be emphasized that APCI is not usually used in protein analysis. In order to verify the applicability of the proposed method to the analysis of proteins, two standard proteins (horse cytochrome c and horse myoglobin) were analyzed. A mixture of the two proteins was also analyzed showing that this novel approach, based on the use of APCI, can be used in the analysis of protein mixtures.

Original languageEnglish
Pages (from-to)1153-1159
Number of pages7
JournalRapid Communications in Mass Spectrometry
Volume16
Issue number12
DOIs
Publication statusPublished - 2002

Fingerprint

Partial discharges
Atmospheric pressure
Ionization
Mass spectrometry
Ions
Proteins
Myoglobin
Cytochromes c
Monitoring

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Cite this

@article{6963d8c6b5e1409dade19033313ecfd3,
title = "Analysis of protein ions in the range 3000-12000 Th under partial (no discharge) atmospheric pressure chemical ionization conditions using ion trap mass spectrometry",
abstract = "A new approach, based on the use of atmospheric pressure chemical ionization ion trap mass spectrometry (APCI-ITMS), but without a corona discharge, was investigated for application to creating and monitoring protein ions. It must be emphasized that APCI is not usually used in protein analysis. In order to verify the applicability of the proposed method to the analysis of proteins, two standard proteins (horse cytochrome c and horse myoglobin) were analyzed. A mixture of the two proteins was also analyzed showing that this novel approach, based on the use of APCI, can be used in the analysis of protein mixtures.",
author = "Simone Cristoni and Bernardi, {Luigi Rossi} and Ida Biunno and Federico Guidugli",
year = "2002",
doi = "10.1002/rcm.693",
language = "English",
volume = "16",
pages = "1153--1159",
journal = "Rapid Communications in Mass Spectrometry",
issn = "0951-4198",
publisher = "John Wiley and Sons Ltd",
number = "12",

}

TY - JOUR

T1 - Analysis of protein ions in the range 3000-12000 Th under partial (no discharge) atmospheric pressure chemical ionization conditions using ion trap mass spectrometry

AU - Cristoni, Simone

AU - Bernardi, Luigi Rossi

AU - Biunno, Ida

AU - Guidugli, Federico

PY - 2002

Y1 - 2002

N2 - A new approach, based on the use of atmospheric pressure chemical ionization ion trap mass spectrometry (APCI-ITMS), but without a corona discharge, was investigated for application to creating and monitoring protein ions. It must be emphasized that APCI is not usually used in protein analysis. In order to verify the applicability of the proposed method to the analysis of proteins, two standard proteins (horse cytochrome c and horse myoglobin) were analyzed. A mixture of the two proteins was also analyzed showing that this novel approach, based on the use of APCI, can be used in the analysis of protein mixtures.

AB - A new approach, based on the use of atmospheric pressure chemical ionization ion trap mass spectrometry (APCI-ITMS), but without a corona discharge, was investigated for application to creating and monitoring protein ions. It must be emphasized that APCI is not usually used in protein analysis. In order to verify the applicability of the proposed method to the analysis of proteins, two standard proteins (horse cytochrome c and horse myoglobin) were analyzed. A mixture of the two proteins was also analyzed showing that this novel approach, based on the use of APCI, can be used in the analysis of protein mixtures.

UR - http://www.scopus.com/inward/record.url?scp=0035983402&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035983402&partnerID=8YFLogxK

U2 - 10.1002/rcm.693

DO - 10.1002/rcm.693

M3 - Article

C2 - 12112265

AN - SCOPUS:0035983402

VL - 16

SP - 1153

EP - 1159

JO - Rapid Communications in Mass Spectrometry

JF - Rapid Communications in Mass Spectrometry

SN - 0951-4198

IS - 12

ER -