TY - JOUR
T1 - Analysis of SAS gene and CDK4 and MDM2 proteins in low-grade osteosarcoma
AU - Ragazzini, P.
AU - Gamberi, G.
AU - Benassi, M. S.
AU - Orlando, C.
AU - Sestini, R.
AU - Ferrari, C.
AU - Molendini, L.
AU - Sollazzo, R.
AU - Merli, M.
AU - Magagnoli, G.
AU - Bertoni, F.
AU - Bohling, T.
AU - Pazzagli, M.
AU - Picci, P.
PY - 1999
Y1 - 1999
N2 - The region q13-15 of chromosome 12 contains SAS, CDK4, and MDM2 genes that are rearranged or amplified in a variety of human sarcomas. This study evaluated SAS gene amplification, and MDM2 and CDK4 protein expression in 20 tumor samples of central low-grade osteosarcoma (16 primary, 3 recurrences, 1 lung metastasis). SAS amplification was analyzed by quantitative polymerase chain reaction (PCR), while from the same paraffin-embedded samples, MDM2 and CDK4 protein expression was evaluated by immunohistochemistry. MDM2 and CDK4 proteins were found strongly expressed in 35% and 65%, respectively, of the samples. SAS was found amplified in 15% of the samples. These findings indicate that these genes may be involved in tumorigenesis and progression of low-grade osteosarcoma.
AB - The region q13-15 of chromosome 12 contains SAS, CDK4, and MDM2 genes that are rearranged or amplified in a variety of human sarcomas. This study evaluated SAS gene amplification, and MDM2 and CDK4 protein expression in 20 tumor samples of central low-grade osteosarcoma (16 primary, 3 recurrences, 1 lung metastasis). SAS amplification was analyzed by quantitative polymerase chain reaction (PCR), while from the same paraffin-embedded samples, MDM2 and CDK4 protein expression was evaluated by immunohistochemistry. MDM2 and CDK4 proteins were found strongly expressed in 35% and 65%, respectively, of the samples. SAS was found amplified in 15% of the samples. These findings indicate that these genes may be involved in tumorigenesis and progression of low-grade osteosarcoma.
KW - Low-grade osteosarcoma
KW - Oncogene
KW - Quantitative PCR
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U2 - 10.1046/j.1525-1500.1999.09907.x
DO - 10.1046/j.1525-1500.1999.09907.x
M3 - Article
C2 - 10101594
AN - SCOPUS:0032991072
VL - 23
SP - 129
EP - 136
JO - Cancer Detection and Prevention
JF - Cancer Detection and Prevention
SN - 0361-090X
IS - 2
ER -