Analysis of SAS gene and CDK4 and MDM2 proteins in low-grade osteosarcoma

P. Ragazzini, G. Gamberi, M. S. Benassi, C. Orlando, R. Sestini, C. Ferrari, L. Molendini, R. Sollazzo, M. Merli, G. Magagnoli, F. Bertoni, T. Bohling, M. Pazzagli, P. Picci

Research output: Contribution to journalArticle

Abstract

The region q13-15 of chromosome 12 contains SAS, CDK4, and MDM2 genes that are rearranged or amplified in a variety of human sarcomas. This study evaluated SAS gene amplification, and MDM2 and CDK4 protein expression in 20 tumor samples of central low-grade osteosarcoma (16 primary, 3 recurrences, 1 lung metastasis). SAS amplification was analyzed by quantitative polymerase chain reaction (PCR), while from the same paraffin-embedded samples, MDM2 and CDK4 protein expression was evaluated by immunohistochemistry. MDM2 and CDK4 proteins were found strongly expressed in 35% and 65%, respectively, of the samples. SAS was found amplified in 15% of the samples. These findings indicate that these genes may be involved in tumorigenesis and progression of low-grade osteosarcoma.

Original languageEnglish
Pages (from-to)129-136
Number of pages8
JournalCancer Detection and Prevention
Volume23
Issue number2
DOIs
Publication statusPublished - 1999

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Keywords

  • Low-grade osteosarcoma
  • Oncogene
  • Quantitative PCR

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Ragazzini, P., Gamberi, G., Benassi, M. S., Orlando, C., Sestini, R., Ferrari, C., Molendini, L., Sollazzo, R., Merli, M., Magagnoli, G., Bertoni, F., Bohling, T., Pazzagli, M., & Picci, P. (1999). Analysis of SAS gene and CDK4 and MDM2 proteins in low-grade osteosarcoma. Cancer Detection and Prevention, 23(2), 129-136. https://doi.org/10.1046/j.1525-1500.1999.09907.x