Analysis of SAS gene and CDK4 and MDM2 proteins in low-grade osteosarcoma

P. Ragazzini; G. Gamberi; M. S. Benassi; C. Orlando; R. Sestini; C. Ferrari; L. Molendini; R. Sollazzo; M. Merli; G. Magagnoli; F. Bertoni; T. Bohling; M. Pazzagli; P. Picci

doi:10.1046/j.1525-1500.1999.09907.x

Analysis of SAS gene and CDK4 and MDM2 proteins in low-grade osteosarcoma

P. Ragazzini, G. Gamberi, M. S. Benassi, C. Orlando, R. Sestini, C. Ferrari, L. Molendini, R. Sollazzo, M. Merli, G. Magagnoli, F. Bertoni, T. Bohling, M. Pazzagli, P. Picci

Istituti Ortopedici Rizzoli

Research output: Contribution to journal › Article › peer-review

Abstract

The region q13-15 of chromosome 12 contains SAS, CDK4, and MDM2 genes that are rearranged or amplified in a variety of human sarcomas. This study evaluated SAS gene amplification, and MDM2 and CDK4 protein expression in 20 tumor samples of central low-grade osteosarcoma (16 primary, 3 recurrences, 1 lung metastasis). SAS amplification was analyzed by quantitative polymerase chain reaction (PCR), while from the same paraffin-embedded samples, MDM2 and CDK4 protein expression was evaluated by immunohistochemistry. MDM2 and CDK4 proteins were found strongly expressed in 35% and 65%, respectively, of the samples. SAS was found amplified in 15% of the samples. These findings indicate that these genes may be involved in tumorigenesis and progression of low-grade osteosarcoma.

Original language	English
Pages (from-to)	129-136
Number of pages	8
Journal	Cancer Detection and Prevention
Volume	23
Issue number	2
DOIs	https://doi.org/10.1046/j.1525-1500.1999.09907.x
Publication status	Published - 1999

Keywords

Low-grade osteosarcoma
Oncogene
Quantitative PCR

ASJC Scopus subject areas

Cancer Research
Oncology

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Ragazzini, P., Gamberi, G., Benassi, M. S., Orlando, C., Sestini, R., Ferrari, C., Molendini, L., Sollazzo, R., Merli, M., Magagnoli, G., Bertoni, F., Bohling, T., Pazzagli, M., & Picci, P. (1999). Analysis of SAS gene and CDK4 and MDM2 proteins in low-grade osteosarcoma. Cancer Detection and Prevention, 23(2), 129-136. https://doi.org/10.1046/j.1525-1500.1999.09907.x

Analysis of SAS gene and CDK4 and MDM2 proteins in low-grade osteosarcoma. / Ragazzini, P.; Gamberi, G.; Benassi, M. S.; Orlando, C.; Sestini, R.; Ferrari, C.; Molendini, L.; Sollazzo, R.; Merli, M.; Magagnoli, G.; Bertoni, F.; Bohling, T.; Pazzagli, M.; Picci, P.

In: Cancer Detection and Prevention, Vol. 23, No. 2, 1999, p. 129-136.

Research output: Contribution to journal › Article › peer-review

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abstract = "The region q13-15 of chromosome 12 contains SAS, CDK4, and MDM2 genes that are rearranged or amplified in a variety of human sarcomas. This study evaluated SAS gene amplification, and MDM2 and CDK4 protein expression in 20 tumor samples of central low-grade osteosarcoma (16 primary, 3 recurrences, 1 lung metastasis). SAS amplification was analyzed by quantitative polymerase chain reaction (PCR), while from the same paraffin-embedded samples, MDM2 and CDK4 protein expression was evaluated by immunohistochemistry. MDM2 and CDK4 proteins were found strongly expressed in 35% and 65%, respectively, of the samples. SAS was found amplified in 15% of the samples. These findings indicate that these genes may be involved in tumorigenesis and progression of low-grade osteosarcoma.",

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AU - Sestini, R.

AU - Ferrari, C.

AU - Molendini, L.

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AU - Merli, M.

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AU - Bertoni, F.

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