Analysis of signal transducing mechanisms in CD3+ CD4- CD8- cells expressing the putative T cell receptor γ gene product

G. Pantaleo, S. Ferrini, M. R. Zocchi, C. Bottino, R. Biassoni, L. Moretta, A. Moretta

Research output: Contribution to journalArticle

Abstract

The signal transducing mechanisms, involved in the activation of CD3+ WT31- cells bearing the putative products of T cell receptor γ genes, have been investigated. After stimulation with phytohemagglutinin or with monoclonal antibodies directed against CD2 or CD3 surface molecules, a rapid increase in free cytoplasmic Ca2+ concentration was detected in one representative CD3+ WT31- clone and in PEER cell line. Experiments performed in the presence of the Ca2+ chelator EGTA indicated that the free cytoplasmic Ca2+ concentration rise was consequent to an early release of Ca2+ from internal stores, followed by a sustained Ca2+ influx from the extracellular compartment. Moreover, increased levels of inositol-3-phosphate (the putative mobilizer of Ca2+ from the intracellular stores) were observed after stimulation, thus suggesting that activation of this cell subset occurs via the classical inositol-lipid metabolic pathway.

Original languageEnglish
Pages (from-to)3580-3584
Number of pages5
JournalJournal of Immunology
Volume139
Issue number11
Publication statusPublished - 1987

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ASJC Scopus subject areas

  • Immunology

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