Analysis of single, purified inclusions as a novel approach to understand methamphetamine neurotoxicity

Paola Lenzi, Federica Fulceri, Gloria Lazzeri, Alessandro Casini, Stefano Ruggieri, Antonio Paparelli, Francesco Fornai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A variety of neurodegenerative diseases leading to movement disorders such as Parkinson's disease (PD) are characterized by neuronal inclusions. Despite evidence of the presence of these intrusions, these intracellular bodies have been poorly investigated because of the technical limits of reproducing them in experimental models and the difficulties in isolating these ultrastuctures. Here, we describe a simple method for the isolation of single, purified inclusion bodies using immunomagnetic separation. We profited from the high number and maturation stage of inclusions produced in vitro by methamphetamine (METH) in cultured PC12 cells; in fact, this experimental condition is highly reproducible and has a limited number of experimental variables, while it is predictive of what is described in vivo in dopamine neurons.

Original languageEnglish
Title of host publicationAnnals of the New York Academy of Sciences
Pages186-190
Number of pages5
Volume1139
DOIs
Publication statusPublished - Oct 2008

Publication series

NameAnnals of the New York Academy of Sciences
Volume1139
ISSN (Print)00778923
ISSN (Electronic)17496632

Keywords

  • Inclusion purification
  • Inclusion structure
  • Magnetic beads
  • Neurodegeneration

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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