TY - CHAP
T1 - Analysis of T cell activation by confocal microscopy
AU - Larghi, Paola
AU - Viola, Antonella
AU - Molon, Barbara
PY - 2017
Y1 - 2017
N2 - Antigen-recognition by T cells requires the physical association with an antigen presenting cell (APC). At the interface between a T cell and an APC, the orchestrated redistribution of lipids, membrane receptors, and intracellular adaptors assembles a highly specialized junction, controlling the communication between the two cells, named the immunologic synapse (IS). The proper organization of the IS is a key step in host defense. Indeed, an appropriate T cell–APC interaction ensures the elimination of a wide range of pathogens and aberrant cells, whereas a deregulated IS formation leads to pathological situations such as infections, tumor development, or autoimmunity. Over the last decades T-cell scientists pioneered new imaging approaches to investigate IS assembly and organization. Microscopy techniques enable researchers to directly monitor, in space and time, the dynamics regulating T cell activation. In this chapter, we describe in detail different microscopy protocols to visualize and analyze the recruitment of different molecules and organelles at the IS.
AB - Antigen-recognition by T cells requires the physical association with an antigen presenting cell (APC). At the interface between a T cell and an APC, the orchestrated redistribution of lipids, membrane receptors, and intracellular adaptors assembles a highly specialized junction, controlling the communication between the two cells, named the immunologic synapse (IS). The proper organization of the IS is a key step in host defense. Indeed, an appropriate T cell–APC interaction ensures the elimination of a wide range of pathogens and aberrant cells, whereas a deregulated IS formation leads to pathological situations such as infections, tumor development, or autoimmunity. Over the last decades T-cell scientists pioneered new imaging approaches to investigate IS assembly and organization. Microscopy techniques enable researchers to directly monitor, in space and time, the dynamics regulating T cell activation. In this chapter, we describe in detail different microscopy protocols to visualize and analyze the recruitment of different molecules and organelles at the IS.
KW - Antigen presenting cells
KW - Calcium flux
KW - Immunologic synapse
KW - Microscopy
KW - T cells
UR - http://www.scopus.com/inward/record.url?scp=84994201567&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84994201567&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-6548-9_5
DO - 10.1007/978-1-4939-6548-9_5
M3 - Chapter
AN - SCOPUS:84994201567
VL - 1514
T3 - Methods in Molecular Biology
SP - 63
EP - 81
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -