Analysis of the antigenic profile of uveal melanoma lesions with anti-cutaneous melanoma-associated antigen and anti-HLA monoclonal antibodies

P. G. Natali, A. Bigotti, M. R. Nicotra, R. M. Nardi, A. Delovu, O. Segatto, S. Ferrone

Research output: Contribution to journalArticle

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Abstract

The reactivity of 12 surgically removed uveal melanoma lesions with monoclonal antibodies (MoAb) to 14 membrane-bound and 2 cytoplasmic cutaneous melanoma-associated antigens (MAA), to the 2 subunits of HLA Class I antigens and to the gene products of the HLA-D region was compared with that of cutaneous melanoma lesions and correlated with their histiotype. The membrane-bound determinants defined by the anti-M(r)92,000 and 45,000 MAA MoAb TP39.1, anti-M(r)110,000 MAA MoAb M111, anti-M(r)118,000 MAA MoAb TP36.1, anti-M(r)115,000 MAA MoAb 345.134, anti-ICAM-1 MoAb CL203.4 and anti-M(r)31,000 MAA MoAb M2590, and the cytoplasmic determinants defined by the anti-MAA MoAb 465.12 and 2G-10 display a distribution in uveal melanoma lesions similar to that in cutaneous melanoma lesions. On the other hand, the membrane-bound determinants defined by the anti-M(r)100,000 MAA MoAb 376.96, anti-9-O-acetyl-G(D)3 ganglioside MoAb ME311 and anti G(D)2-G(D)3 ganglioside MOAb ME361 were not detected in uveal melanoma lesions tested. Furthermore, the membrane-bound determinants defined by the anti-G(D)3 MoAb R24, anti-nerve growth factor receptor MoAb ME20.4, anti-M(r)97,000 MAA MoAb 140.240, anti-carcinoembryonic antigen MoAb B1.1 and anti-HMW-MAA 149.53, 225.28, and 763.74 have a markedly lower expression in uveal than in cutaneous melanoma lesions. Incubation of uveal melanoma lesions with the pool of the MoAb 149.53, 225.28, and 763.74 recognizing distinct and spatially distant determinants of te HMW-MAA increased the intensity of staining of six lesions and stained four lesions which were not stained by the individual monoclonal antibodies. The distribution of HLA Class I antigens in uveal melanoma lesions resembles that in cutaneous melanoma lesions, since they are expressed in all the lesions of the mixed and epithelioid type but were not detected in those of the spindle type, i.e., the counterparts of nevocellular nevi. HLA Class II antigens are expressed with a lower frequency in uveal than in cutaneous melanoma lesions, since they were detected only in 2 of the 12 lesions. One of them is of the mixed types and the other one of the epithelioid type. Besides HLA antigens the determinants defined by the anti-carcinoembryonic MoAb B1.1, anti-ICAM-1 MoAb CL203.4, and anti G(D)3 MoAb R24 displayed a differential distribution in the different histiotypes of uveal melanoma, since they are preferentially expressed in lesions of the mixed and epithelioid type.

Original languageEnglish
Pages (from-to)1269-1274
Number of pages6
JournalCancer Research
Volume49
Issue number5
Publication statusPublished - 1989

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Melanoma-Specific Antigens
Monoclonal Antibodies
Skin
Melanoma
HLA Antigens
Uveal melanoma
Histocompatibility Antigens Class I
Membranes
Gangliosides
Intercellular Adhesion Molecule-1
HLA-D Antigens
Nerve Growth Factor Receptor

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Natali, P. G., Bigotti, A., Nicotra, M. R., Nardi, R. M., Delovu, A., Segatto, O., & Ferrone, S. (1989). Analysis of the antigenic profile of uveal melanoma lesions with anti-cutaneous melanoma-associated antigen and anti-HLA monoclonal antibodies. Cancer Research, 49(5), 1269-1274.

Analysis of the antigenic profile of uveal melanoma lesions with anti-cutaneous melanoma-associated antigen and anti-HLA monoclonal antibodies. / Natali, P. G.; Bigotti, A.; Nicotra, M. R.; Nardi, R. M.; Delovu, A.; Segatto, O.; Ferrone, S.

In: Cancer Research, Vol. 49, No. 5, 1989, p. 1269-1274.

Research output: Contribution to journalArticle

Natali, PG, Bigotti, A, Nicotra, MR, Nardi, RM, Delovu, A, Segatto, O & Ferrone, S 1989, 'Analysis of the antigenic profile of uveal melanoma lesions with anti-cutaneous melanoma-associated antigen and anti-HLA monoclonal antibodies', Cancer Research, vol. 49, no. 5, pp. 1269-1274.
Natali, P. G. ; Bigotti, A. ; Nicotra, M. R. ; Nardi, R. M. ; Delovu, A. ; Segatto, O. ; Ferrone, S. / Analysis of the antigenic profile of uveal melanoma lesions with anti-cutaneous melanoma-associated antigen and anti-HLA monoclonal antibodies. In: Cancer Research. 1989 ; Vol. 49, No. 5. pp. 1269-1274.
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abstract = "The reactivity of 12 surgically removed uveal melanoma lesions with monoclonal antibodies (MoAb) to 14 membrane-bound and 2 cytoplasmic cutaneous melanoma-associated antigens (MAA), to the 2 subunits of HLA Class I antigens and to the gene products of the HLA-D region was compared with that of cutaneous melanoma lesions and correlated with their histiotype. The membrane-bound determinants defined by the anti-M(r)92,000 and 45,000 MAA MoAb TP39.1, anti-M(r)110,000 MAA MoAb M111, anti-M(r)118,000 MAA MoAb TP36.1, anti-M(r)115,000 MAA MoAb 345.134, anti-ICAM-1 MoAb CL203.4 and anti-M(r)31,000 MAA MoAb M2590, and the cytoplasmic determinants defined by the anti-MAA MoAb 465.12 and 2G-10 display a distribution in uveal melanoma lesions similar to that in cutaneous melanoma lesions. On the other hand, the membrane-bound determinants defined by the anti-M(r)100,000 MAA MoAb 376.96, anti-9-O-acetyl-G(D)3 ganglioside MoAb ME311 and anti G(D)2-G(D)3 ganglioside MOAb ME361 were not detected in uveal melanoma lesions tested. Furthermore, the membrane-bound determinants defined by the anti-G(D)3 MoAb R24, anti-nerve growth factor receptor MoAb ME20.4, anti-M(r)97,000 MAA MoAb 140.240, anti-carcinoembryonic antigen MoAb B1.1 and anti-HMW-MAA 149.53, 225.28, and 763.74 have a markedly lower expression in uveal than in cutaneous melanoma lesions. Incubation of uveal melanoma lesions with the pool of the MoAb 149.53, 225.28, and 763.74 recognizing distinct and spatially distant determinants of te HMW-MAA increased the intensity of staining of six lesions and stained four lesions which were not stained by the individual monoclonal antibodies. The distribution of HLA Class I antigens in uveal melanoma lesions resembles that in cutaneous melanoma lesions, since they are expressed in all the lesions of the mixed and epithelioid type but were not detected in those of the spindle type, i.e., the counterparts of nevocellular nevi. HLA Class II antigens are expressed with a lower frequency in uveal than in cutaneous melanoma lesions, since they were detected only in 2 of the 12 lesions. One of them is of the mixed types and the other one of the epithelioid type. Besides HLA antigens the determinants defined by the anti-carcinoembryonic MoAb B1.1, anti-ICAM-1 MoAb CL203.4, and anti G(D)3 MoAb R24 displayed a differential distribution in the different histiotypes of uveal melanoma, since they are preferentially expressed in lesions of the mixed and epithelioid type.",
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T1 - Analysis of the antigenic profile of uveal melanoma lesions with anti-cutaneous melanoma-associated antigen and anti-HLA monoclonal antibodies

AU - Natali, P. G.

AU - Bigotti, A.

AU - Nicotra, M. R.

AU - Nardi, R. M.

AU - Delovu, A.

AU - Segatto, O.

AU - Ferrone, S.

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N2 - The reactivity of 12 surgically removed uveal melanoma lesions with monoclonal antibodies (MoAb) to 14 membrane-bound and 2 cytoplasmic cutaneous melanoma-associated antigens (MAA), to the 2 subunits of HLA Class I antigens and to the gene products of the HLA-D region was compared with that of cutaneous melanoma lesions and correlated with their histiotype. The membrane-bound determinants defined by the anti-M(r)92,000 and 45,000 MAA MoAb TP39.1, anti-M(r)110,000 MAA MoAb M111, anti-M(r)118,000 MAA MoAb TP36.1, anti-M(r)115,000 MAA MoAb 345.134, anti-ICAM-1 MoAb CL203.4 and anti-M(r)31,000 MAA MoAb M2590, and the cytoplasmic determinants defined by the anti-MAA MoAb 465.12 and 2G-10 display a distribution in uveal melanoma lesions similar to that in cutaneous melanoma lesions. On the other hand, the membrane-bound determinants defined by the anti-M(r)100,000 MAA MoAb 376.96, anti-9-O-acetyl-G(D)3 ganglioside MoAb ME311 and anti G(D)2-G(D)3 ganglioside MOAb ME361 were not detected in uveal melanoma lesions tested. Furthermore, the membrane-bound determinants defined by the anti-G(D)3 MoAb R24, anti-nerve growth factor receptor MoAb ME20.4, anti-M(r)97,000 MAA MoAb 140.240, anti-carcinoembryonic antigen MoAb B1.1 and anti-HMW-MAA 149.53, 225.28, and 763.74 have a markedly lower expression in uveal than in cutaneous melanoma lesions. Incubation of uveal melanoma lesions with the pool of the MoAb 149.53, 225.28, and 763.74 recognizing distinct and spatially distant determinants of te HMW-MAA increased the intensity of staining of six lesions and stained four lesions which were not stained by the individual monoclonal antibodies. The distribution of HLA Class I antigens in uveal melanoma lesions resembles that in cutaneous melanoma lesions, since they are expressed in all the lesions of the mixed and epithelioid type but were not detected in those of the spindle type, i.e., the counterparts of nevocellular nevi. HLA Class II antigens are expressed with a lower frequency in uveal than in cutaneous melanoma lesions, since they were detected only in 2 of the 12 lesions. One of them is of the mixed types and the other one of the epithelioid type. Besides HLA antigens the determinants defined by the anti-carcinoembryonic MoAb B1.1, anti-ICAM-1 MoAb CL203.4, and anti G(D)3 MoAb R24 displayed a differential distribution in the different histiotypes of uveal melanoma, since they are preferentially expressed in lesions of the mixed and epithelioid type.

AB - The reactivity of 12 surgically removed uveal melanoma lesions with monoclonal antibodies (MoAb) to 14 membrane-bound and 2 cytoplasmic cutaneous melanoma-associated antigens (MAA), to the 2 subunits of HLA Class I antigens and to the gene products of the HLA-D region was compared with that of cutaneous melanoma lesions and correlated with their histiotype. The membrane-bound determinants defined by the anti-M(r)92,000 and 45,000 MAA MoAb TP39.1, anti-M(r)110,000 MAA MoAb M111, anti-M(r)118,000 MAA MoAb TP36.1, anti-M(r)115,000 MAA MoAb 345.134, anti-ICAM-1 MoAb CL203.4 and anti-M(r)31,000 MAA MoAb M2590, and the cytoplasmic determinants defined by the anti-MAA MoAb 465.12 and 2G-10 display a distribution in uveal melanoma lesions similar to that in cutaneous melanoma lesions. On the other hand, the membrane-bound determinants defined by the anti-M(r)100,000 MAA MoAb 376.96, anti-9-O-acetyl-G(D)3 ganglioside MoAb ME311 and anti G(D)2-G(D)3 ganglioside MOAb ME361 were not detected in uveal melanoma lesions tested. Furthermore, the membrane-bound determinants defined by the anti-G(D)3 MoAb R24, anti-nerve growth factor receptor MoAb ME20.4, anti-M(r)97,000 MAA MoAb 140.240, anti-carcinoembryonic antigen MoAb B1.1 and anti-HMW-MAA 149.53, 225.28, and 763.74 have a markedly lower expression in uveal than in cutaneous melanoma lesions. Incubation of uveal melanoma lesions with the pool of the MoAb 149.53, 225.28, and 763.74 recognizing distinct and spatially distant determinants of te HMW-MAA increased the intensity of staining of six lesions and stained four lesions which were not stained by the individual monoclonal antibodies. The distribution of HLA Class I antigens in uveal melanoma lesions resembles that in cutaneous melanoma lesions, since they are expressed in all the lesions of the mixed and epithelioid type but were not detected in those of the spindle type, i.e., the counterparts of nevocellular nevi. HLA Class II antigens are expressed with a lower frequency in uveal than in cutaneous melanoma lesions, since they were detected only in 2 of the 12 lesions. One of them is of the mixed types and the other one of the epithelioid type. Besides HLA antigens the determinants defined by the anti-carcinoembryonic MoAb B1.1, anti-ICAM-1 MoAb CL203.4, and anti G(D)3 MoAb R24 displayed a differential distribution in the different histiotypes of uveal melanoma, since they are preferentially expressed in lesions of the mixed and epithelioid type.

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