Analysis of the dynamics of cryoaggregation by light-scattering spectrometry

Enrico Di Stasio, Patrizia Bizzarri, Maurizio Bove, Milvia Casato, Bruno Giardina, Massimo Fiorilli, Antonio Galtieri, Leopoldo P. Pucillo

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Cryoglobulins are proteins that precipitate at temperatures below 37 °C. Cold-induced precipitation of proteins may occur in vivo secondary to several important diseases, and lead to pathological manifestations involving different organs. Cryoprecipitation may be observed in vitro by exposing serum samples, supposed to contain cryoglobulins, to low temperatures, but this needs several days to occur. Protein-protein interactions leading to cryoprecipitation are still poorly understood and the knowledge of the underlying mechanism may be relevant to the understanding of the onset of pathological manifestations. Using light-scattering spectrometry, we studied cryoprecipitation occurring in vitro at different temperatures and cryoglobulin concentrations. We describe the kinetics of the cold-induced precipitation of mixed cryoglobulins, measured as increase in turbidity. The plots obtained demonstrate that the cryoprecipitation did not occur as a single-step reaction, but consisted of four distinct phases where both temperature and cryoglobulin concentration affected the immune complexes formation. Light scatter spectrometry may provide a simple, sensitive and rapid method for the detection of cryoglobulins.

Original languageEnglish
Pages (from-to)152-158
Number of pages7
JournalClinical Chemistry and Laboratory Medicine
Volume41
Issue number2
DOIs
Publication statusPublished - 2003

Fingerprint

Cryoglobulins
Light scattering
Spectrometry
Spectrum Analysis
Temperature
Proteins
Light
Turbidity
Antigen-Antibody Complex
Dynamic Light Scattering
Precipitates
Kinetics
Serum

Keywords

  • Cryoaggregation
  • Cryoglobulin
  • Immune complexes

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Di Stasio, E., Bizzarri, P., Bove, M., Casato, M., Giardina, B., Fiorilli, M., ... Pucillo, L. P. (2003). Analysis of the dynamics of cryoaggregation by light-scattering spectrometry. Clinical Chemistry and Laboratory Medicine, 41(2), 152-158. https://doi.org/10.1515/CCLM.2003.025

Analysis of the dynamics of cryoaggregation by light-scattering spectrometry. / Di Stasio, Enrico; Bizzarri, Patrizia; Bove, Maurizio; Casato, Milvia; Giardina, Bruno; Fiorilli, Massimo; Galtieri, Antonio; Pucillo, Leopoldo P.

In: Clinical Chemistry and Laboratory Medicine, Vol. 41, No. 2, 2003, p. 152-158.

Research output: Contribution to journalArticle

Di Stasio, E, Bizzarri, P, Bove, M, Casato, M, Giardina, B, Fiorilli, M, Galtieri, A & Pucillo, LP 2003, 'Analysis of the dynamics of cryoaggregation by light-scattering spectrometry', Clinical Chemistry and Laboratory Medicine, vol. 41, no. 2, pp. 152-158. https://doi.org/10.1515/CCLM.2003.025
Di Stasio, Enrico ; Bizzarri, Patrizia ; Bove, Maurizio ; Casato, Milvia ; Giardina, Bruno ; Fiorilli, Massimo ; Galtieri, Antonio ; Pucillo, Leopoldo P. / Analysis of the dynamics of cryoaggregation by light-scattering spectrometry. In: Clinical Chemistry and Laboratory Medicine. 2003 ; Vol. 41, No. 2. pp. 152-158.
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