Analysis of the human CD36 leucocyte differentiation antigen by means of the monoclonal antibody NL07

M. Alessio, D. Ghigo, G. Garbarino, M. Geuna, F. Malavasi

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The murine monoclonal antibody (MoAb) NL07 was generated by immunization with human platelet extracts. NL07 MoAb recognized a molecule expressed by human platelets, monocytes, and endothelial cells, as well as by the myelomonocytic line U937 and by some melanoma cells or lines. Normal endothelial cells and the melanoma cells express the NL07 epitope only while adhering to a substrate. SDS-polyacrylamide gel electrophoresis and two-dimensional gel analysis indicate that the molecule recognized by NL07 MoAb on platelets is a single chain structure featuring a molecular weight of 85 kDa under reducing conditions, with an acidic isoelectric point ranging from 5.2 to 5.5. The specific phenotype distribution and the biochemical structure indicate that NL07 MoAb recognizes the platelet GPIV (CD36) molecule, a surface glycoprotein with a functional role of thrombospondin receptor. The results of competition tests with OKM5 MoAb (specific for the CD36 molecule) confirm the molecular specificity and epitope coincidence. Furthermore, upon binding to the platelets, NL07 MoAb is able to transmit via CD36 an activation signal which is followed by a potent aggregation. On the contrary, there is lack of evidence concerning the ability of the CD36 molecule of transmitting signal(s) on the U937 cells.

Original languageEnglish
Pages (from-to)487-500
Number of pages14
JournalCellular Immunology
Volume137
Issue number2
DOIs
Publication statusPublished - Oct 15 1991

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CD Antigens
CD36 Antigens
Monoclonal Antibodies
Blood Platelets
Epitopes
Melanoma
Endothelial Cells
U937 Cells
Membrane Glycoproteins
Electrophoresis, Gel, Two-Dimensional
Isoelectric Point
Polyacrylamide Gel Electrophoresis
Monocytes
Immunization
Molecular Weight
Phenotype
Cell Line

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Analysis of the human CD36 leucocyte differentiation antigen by means of the monoclonal antibody NL07. / Alessio, M.; Ghigo, D.; Garbarino, G.; Geuna, M.; Malavasi, F.

In: Cellular Immunology, Vol. 137, No. 2, 15.10.1991, p. 487-500.

Research output: Contribution to journalArticle

Alessio, M. ; Ghigo, D. ; Garbarino, G. ; Geuna, M. ; Malavasi, F. / Analysis of the human CD36 leucocyte differentiation antigen by means of the monoclonal antibody NL07. In: Cellular Immunology. 1991 ; Vol. 137, No. 2. pp. 487-500.
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