Abstract
Background: Classical Kaposi's Sarcoma (cKS) is a rare vascular tumor, which develops in subjects infected with Human Herpesvirus-8 (HHV-8). Beside the host predisposing factors, viral genetic variants might possibly be related to disease development. The aim of this study was to identify HHV-8 variants in patients with cKS or in HHV-8 infected subjects either asymptomatic or with cKS-unrelated cutaneous lymphoproliferative disorders. Methods: The VR1 and VR2 regions of the ORF K1 sequence were analyzed in samples (peripheral blood and/or lesional tissue) collected between 2000 and 2010 from 27 subjects with HHV-8 infection, established by the presence of anti-HHV-8 antibodies. On the basis of viral genotyping, a phylogenetic analysis and a time-scaled evaluation were performed. Results: Two main clades of HHV-8, corresponding to A and C subtypes, were identified. Moreover, for each subtype, two main clusters were found distinctively associated to cKS or non-cKS subjects. Selective pressure analysis showed twelve sites of the K1 coding gene (VR1 and VR2 regions) under positive selective pressure and one site under negative pressure. Conclusion: Thus, present data suggest that HHV-8 genetic variants may influence the susceptibility to cKS in individuals with HHV-8 infection.
| Original language | English |
|---|---|
| Article number | 1 |
| Journal | Journal of Experimental and Clinical Cancer Research |
| Volume | 34 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2015 |
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Keywords
- HHV-8
- HHV-8 variants
- Kaposi's sarcoma
- Phylogenesis
- Selective pressure
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Analysis of the ORFK1 hypervariable regions reveal distinct HHV-8 clustering in Kaposi's sarcoma and non-Kaposi's cases. / Cordiali-Fei, Paola; Trento, Elisabetta; Giovanetti, Marta; Lo Presti, Alessandra; Latini, Alessandra; Giuliani, Massimo; D'Agosto, Giovanna; Bordignon, Valentina; Cella, Eleonora; Farchi, Francesca; Ferraro, Carmela; La Parola, Ilaria Lesnoni; Cota, Carlo; Sperduti, Isabella; Vento, Antonella; Cristaudo, Antonio; Ciccozzi, Massimo; Ensoli, Fabrizio.
In: Journal of Experimental and Clinical Cancer Research, Vol. 34, No. 1, 1, 2015.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Analysis of the ORFK1 hypervariable regions reveal distinct HHV-8 clustering in Kaposi's sarcoma and non-Kaposi's cases
AU - Cordiali-Fei, Paola
AU - Trento, Elisabetta
AU - Giovanetti, Marta
AU - Lo Presti, Alessandra
AU - Latini, Alessandra
AU - Giuliani, Massimo
AU - D'Agosto, Giovanna
AU - Bordignon, Valentina
AU - Cella, Eleonora
AU - Farchi, Francesca
AU - Ferraro, Carmela
AU - La Parola, Ilaria Lesnoni
AU - Cota, Carlo
AU - Sperduti, Isabella
AU - Vento, Antonella
AU - Cristaudo, Antonio
AU - Ciccozzi, Massimo
AU - Ensoli, Fabrizio
PY - 2015
Y1 - 2015
N2 - Background: Classical Kaposi's Sarcoma (cKS) is a rare vascular tumor, which develops in subjects infected with Human Herpesvirus-8 (HHV-8). Beside the host predisposing factors, viral genetic variants might possibly be related to disease development. The aim of this study was to identify HHV-8 variants in patients with cKS or in HHV-8 infected subjects either asymptomatic or with cKS-unrelated cutaneous lymphoproliferative disorders. Methods: The VR1 and VR2 regions of the ORF K1 sequence were analyzed in samples (peripheral blood and/or lesional tissue) collected between 2000 and 2010 from 27 subjects with HHV-8 infection, established by the presence of anti-HHV-8 antibodies. On the basis of viral genotyping, a phylogenetic analysis and a time-scaled evaluation were performed. Results: Two main clades of HHV-8, corresponding to A and C subtypes, were identified. Moreover, for each subtype, two main clusters were found distinctively associated to cKS or non-cKS subjects. Selective pressure analysis showed twelve sites of the K1 coding gene (VR1 and VR2 regions) under positive selective pressure and one site under negative pressure. Conclusion: Thus, present data suggest that HHV-8 genetic variants may influence the susceptibility to cKS in individuals with HHV-8 infection.
AB - Background: Classical Kaposi's Sarcoma (cKS) is a rare vascular tumor, which develops in subjects infected with Human Herpesvirus-8 (HHV-8). Beside the host predisposing factors, viral genetic variants might possibly be related to disease development. The aim of this study was to identify HHV-8 variants in patients with cKS or in HHV-8 infected subjects either asymptomatic or with cKS-unrelated cutaneous lymphoproliferative disorders. Methods: The VR1 and VR2 regions of the ORF K1 sequence were analyzed in samples (peripheral blood and/or lesional tissue) collected between 2000 and 2010 from 27 subjects with HHV-8 infection, established by the presence of anti-HHV-8 antibodies. On the basis of viral genotyping, a phylogenetic analysis and a time-scaled evaluation were performed. Results: Two main clades of HHV-8, corresponding to A and C subtypes, were identified. Moreover, for each subtype, two main clusters were found distinctively associated to cKS or non-cKS subjects. Selective pressure analysis showed twelve sites of the K1 coding gene (VR1 and VR2 regions) under positive selective pressure and one site under negative pressure. Conclusion: Thus, present data suggest that HHV-8 genetic variants may influence the susceptibility to cKS in individuals with HHV-8 infection.
KW - HHV-8
KW - HHV-8 variants
KW - Kaposi's sarcoma
KW - Phylogenesis
KW - Selective pressure
UR - http://www.scopus.com/inward/record.url?scp=84971596931&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84971596931&partnerID=8YFLogxK
U2 - 10.1186/s13046-014-0119-0
DO - 10.1186/s13046-014-0119-0
M3 - Article
C2 - 25592960
AN - SCOPUS:84971596931
VL - 34
JO - Journal of Experimental and Clinical Cancer Research
JF - Journal of Experimental and Clinical Cancer Research
SN - 0392-9078
IS - 1
M1 - 1
ER -