Analysis of the ORFK1 hypervariable regions reveal distinct HHV-8 clustering in Kaposi's sarcoma and non-Kaposi's cases

Paola Cordiali-Fei, Elisabetta Trento, Marta Giovanetti, Alessandra Lo Presti, Alessandra Latini, Massimo Giuliani, Giovanna D'Agosto, Valentina Bordignon, Eleonora Cella, Francesca Farchi, Carmela Ferraro, Ilaria Lesnoni La Parola, Carlo Cota, Isabella Sperduti, Antonella Vento, Antonio Cristaudo, Massimo Ciccozzi, Fabrizio Ensoli

Research output: Contribution to journalArticle

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Abstract

Background: Classical Kaposi's Sarcoma (cKS) is a rare vascular tumor, which develops in subjects infected with Human Herpesvirus-8 (HHV-8). Beside the host predisposing factors, viral genetic variants might possibly be related to disease development. The aim of this study was to identify HHV-8 variants in patients with cKS or in HHV-8 infected subjects either asymptomatic or with cKS-unrelated cutaneous lymphoproliferative disorders. Methods: The VR1 and VR2 regions of the ORF K1 sequence were analyzed in samples (peripheral blood and/or lesional tissue) collected between 2000 and 2010 from 27 subjects with HHV-8 infection, established by the presence of anti-HHV-8 antibodies. On the basis of viral genotyping, a phylogenetic analysis and a time-scaled evaluation were performed. Results: Two main clades of HHV-8, corresponding to A and C subtypes, were identified. Moreover, for each subtype, two main clusters were found distinctively associated to cKS or non-cKS subjects. Selective pressure analysis showed twelve sites of the K1 coding gene (VR1 and VR2 regions) under positive selective pressure and one site under negative pressure. Conclusion: Thus, present data suggest that HHV-8 genetic variants may influence the susceptibility to cKS in individuals with HHV-8 infection.

Original languageEnglish
Article number1
JournalJournal of Experimental and Clinical Cancer Research
Volume34
Issue number1
DOIs
Publication statusPublished - 2015

Fingerprint

Human Herpesvirus 8
Kaposi's Sarcoma
Cluster Analysis
Herpesviridae Infections
Lymphoproliferative Disorders
Causality
Open Reading Frames
Blood Vessels
Pressure
Skin
Antibodies

Keywords

  • HHV-8
  • HHV-8 variants
  • Kaposi's sarcoma
  • Phylogenesis
  • Selective pressure

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Analysis of the ORFK1 hypervariable regions reveal distinct HHV-8 clustering in Kaposi's sarcoma and non-Kaposi's cases. / Cordiali-Fei, Paola; Trento, Elisabetta; Giovanetti, Marta; Lo Presti, Alessandra; Latini, Alessandra; Giuliani, Massimo; D'Agosto, Giovanna; Bordignon, Valentina; Cella, Eleonora; Farchi, Francesca; Ferraro, Carmela; La Parola, Ilaria Lesnoni; Cota, Carlo; Sperduti, Isabella; Vento, Antonella; Cristaudo, Antonio; Ciccozzi, Massimo; Ensoli, Fabrizio.

In: Journal of Experimental and Clinical Cancer Research, Vol. 34, No. 1, 1, 2015.

Research output: Contribution to journalArticle

Cordiali-Fei, P, Trento, E, Giovanetti, M, Lo Presti, A, Latini, A, Giuliani, M, D'Agosto, G, Bordignon, V, Cella, E, Farchi, F, Ferraro, C, La Parola, IL, Cota, C, Sperduti, I, Vento, A, Cristaudo, A, Ciccozzi, M & Ensoli, F 2015, 'Analysis of the ORFK1 hypervariable regions reveal distinct HHV-8 clustering in Kaposi's sarcoma and non-Kaposi's cases', Journal of Experimental and Clinical Cancer Research, vol. 34, no. 1, 1. https://doi.org/10.1186/s13046-014-0119-0
Cordiali-Fei, Paola ; Trento, Elisabetta ; Giovanetti, Marta ; Lo Presti, Alessandra ; Latini, Alessandra ; Giuliani, Massimo ; D'Agosto, Giovanna ; Bordignon, Valentina ; Cella, Eleonora ; Farchi, Francesca ; Ferraro, Carmela ; La Parola, Ilaria Lesnoni ; Cota, Carlo ; Sperduti, Isabella ; Vento, Antonella ; Cristaudo, Antonio ; Ciccozzi, Massimo ; Ensoli, Fabrizio. / Analysis of the ORFK1 hypervariable regions reveal distinct HHV-8 clustering in Kaposi's sarcoma and non-Kaposi's cases. In: Journal of Experimental and Clinical Cancer Research. 2015 ; Vol. 34, No. 1.
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abstract = "Background: Classical Kaposi's Sarcoma (cKS) is a rare vascular tumor, which develops in subjects infected with Human Herpesvirus-8 (HHV-8). Beside the host predisposing factors, viral genetic variants might possibly be related to disease development. The aim of this study was to identify HHV-8 variants in patients with cKS or in HHV-8 infected subjects either asymptomatic or with cKS-unrelated cutaneous lymphoproliferative disorders. Methods: The VR1 and VR2 regions of the ORF K1 sequence were analyzed in samples (peripheral blood and/or lesional tissue) collected between 2000 and 2010 from 27 subjects with HHV-8 infection, established by the presence of anti-HHV-8 antibodies. On the basis of viral genotyping, a phylogenetic analysis and a time-scaled evaluation were performed. Results: Two main clades of HHV-8, corresponding to A and C subtypes, were identified. Moreover, for each subtype, two main clusters were found distinctively associated to cKS or non-cKS subjects. Selective pressure analysis showed twelve sites of the K1 coding gene (VR1 and VR2 regions) under positive selective pressure and one site under negative pressure. Conclusion: Thus, present data suggest that HHV-8 genetic variants may influence the susceptibility to cKS in individuals with HHV-8 infection.",
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AU - Trento, Elisabetta

AU - Giovanetti, Marta

AU - Lo Presti, Alessandra

AU - Latini, Alessandra

AU - Giuliani, Massimo

AU - D'Agosto, Giovanna

AU - Bordignon, Valentina

AU - Cella, Eleonora

AU - Farchi, Francesca

AU - Ferraro, Carmela

AU - La Parola, Ilaria Lesnoni

AU - Cota, Carlo

AU - Sperduti, Isabella

AU - Vento, Antonella

AU - Cristaudo, Antonio

AU - Ciccozzi, Massimo

AU - Ensoli, Fabrizio

PY - 2015

Y1 - 2015

N2 - Background: Classical Kaposi's Sarcoma (cKS) is a rare vascular tumor, which develops in subjects infected with Human Herpesvirus-8 (HHV-8). Beside the host predisposing factors, viral genetic variants might possibly be related to disease development. The aim of this study was to identify HHV-8 variants in patients with cKS or in HHV-8 infected subjects either asymptomatic or with cKS-unrelated cutaneous lymphoproliferative disorders. Methods: The VR1 and VR2 regions of the ORF K1 sequence were analyzed in samples (peripheral blood and/or lesional tissue) collected between 2000 and 2010 from 27 subjects with HHV-8 infection, established by the presence of anti-HHV-8 antibodies. On the basis of viral genotyping, a phylogenetic analysis and a time-scaled evaluation were performed. Results: Two main clades of HHV-8, corresponding to A and C subtypes, were identified. Moreover, for each subtype, two main clusters were found distinctively associated to cKS or non-cKS subjects. Selective pressure analysis showed twelve sites of the K1 coding gene (VR1 and VR2 regions) under positive selective pressure and one site under negative pressure. Conclusion: Thus, present data suggest that HHV-8 genetic variants may influence the susceptibility to cKS in individuals with HHV-8 infection.

AB - Background: Classical Kaposi's Sarcoma (cKS) is a rare vascular tumor, which develops in subjects infected with Human Herpesvirus-8 (HHV-8). Beside the host predisposing factors, viral genetic variants might possibly be related to disease development. The aim of this study was to identify HHV-8 variants in patients with cKS or in HHV-8 infected subjects either asymptomatic or with cKS-unrelated cutaneous lymphoproliferative disorders. Methods: The VR1 and VR2 regions of the ORF K1 sequence were analyzed in samples (peripheral blood and/or lesional tissue) collected between 2000 and 2010 from 27 subjects with HHV-8 infection, established by the presence of anti-HHV-8 antibodies. On the basis of viral genotyping, a phylogenetic analysis and a time-scaled evaluation were performed. Results: Two main clades of HHV-8, corresponding to A and C subtypes, were identified. Moreover, for each subtype, two main clusters were found distinctively associated to cKS or non-cKS subjects. Selective pressure analysis showed twelve sites of the K1 coding gene (VR1 and VR2 regions) under positive selective pressure and one site under negative pressure. Conclusion: Thus, present data suggest that HHV-8 genetic variants may influence the susceptibility to cKS in individuals with HHV-8 infection.

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