mRNA for the α- and β-subunits of mouse β-N-acetylhexosaminidase were detected in tissue sections of epididymis and testis using3H-labelled antisense RNA probes by hybridization in situ. There were clear differences in the intensity of the signals given by the two tissues with α-subunit mRNA being more abundant in testis than epididymis, while the opposite was so with β-subunit mRNA. Differences in the isoenzyme patterns of the two tissues are likely to be due, at least in part, to differences in transcription by the Hexa and Hexb genes.
ASJC Scopus subject areas
- Cell Biology