Analysis of the patterns of expression of mRNAs for the α- and β-subunits of the lysosomal enzyme β-N-acetylhexosaminidase in mouse epididymis and testis

J. L. Stirling, T. Beccari, J. Hoade, F. Pezzetti, M. Calvitti, E. Becchetti, A. Orlacchio

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

mRNA for the α- and β-subunits of mouse β-N-acetylhexosaminidase were detected in tissue sections of epididymis and testis using3H-labelled antisense RNA probes by hybridization in situ. There were clear differences in the intensity of the signals given by the two tissues with α-subunit mRNA being more abundant in testis than epididymis, while the opposite was so with β-subunit mRNA. Differences in the isoenzyme patterns of the two tissues are likely to be due, at least in part, to differences in transcription by the Hexa and Hexb genes.

Original languageEnglish
Pages (from-to)490-494
Number of pages5
JournalHistochemical Journal
Volume23
Issue number10
DOIs
Publication statusPublished - Oct 1991

Fingerprint

Epididymis
Testis
Messenger RNA
Enzymes
RNA Probes
Antisense RNA
Isoenzymes
In Situ Hybridization
Genes

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Analysis of the patterns of expression of mRNAs for the α- and β-subunits of the lysosomal enzyme β-N-acetylhexosaminidase in mouse epididymis and testis. / Stirling, J. L.; Beccari, T.; Hoade, J.; Pezzetti, F.; Calvitti, M.; Becchetti, E.; Orlacchio, A.

In: Histochemical Journal, Vol. 23, No. 10, 10.1991, p. 490-494.

Research output: Contribution to journalArticle

Stirling, J. L. ; Beccari, T. ; Hoade, J. ; Pezzetti, F. ; Calvitti, M. ; Becchetti, E. ; Orlacchio, A. / Analysis of the patterns of expression of mRNAs for the α- and β-subunits of the lysosomal enzyme β-N-acetylhexosaminidase in mouse epididymis and testis. In: Histochemical Journal. 1991 ; Vol. 23, No. 10. pp. 490-494.
@article{55c06bee38364b95ad8a27a188002390,
title = "Analysis of the patterns of expression of mRNAs for the α- and β-subunits of the lysosomal enzyme β-N-acetylhexosaminidase in mouse epididymis and testis",
abstract = "mRNA for the α- and β-subunits of mouse β-N-acetylhexosaminidase were detected in tissue sections of epididymis and testis using3H-labelled antisense RNA probes by hybridization in situ. There were clear differences in the intensity of the signals given by the two tissues with α-subunit mRNA being more abundant in testis than epididymis, while the opposite was so with β-subunit mRNA. Differences in the isoenzyme patterns of the two tissues are likely to be due, at least in part, to differences in transcription by the Hexa and Hexb genes.",
author = "Stirling, {J. L.} and T. Beccari and J. Hoade and F. Pezzetti and M. Calvitti and E. Becchetti and A. Orlacchio",
year = "1991",
month = "10",
doi = "10.1007/BF01041380",
language = "English",
volume = "23",
pages = "490--494",
journal = "Histochemical Journal",
issn = "0018-2214",
publisher = "Kluwer Academic Publishers",
number = "10",

}

TY - JOUR

T1 - Analysis of the patterns of expression of mRNAs for the α- and β-subunits of the lysosomal enzyme β-N-acetylhexosaminidase in mouse epididymis and testis

AU - Stirling, J. L.

AU - Beccari, T.

AU - Hoade, J.

AU - Pezzetti, F.

AU - Calvitti, M.

AU - Becchetti, E.

AU - Orlacchio, A.

PY - 1991/10

Y1 - 1991/10

N2 - mRNA for the α- and β-subunits of mouse β-N-acetylhexosaminidase were detected in tissue sections of epididymis and testis using3H-labelled antisense RNA probes by hybridization in situ. There were clear differences in the intensity of the signals given by the two tissues with α-subunit mRNA being more abundant in testis than epididymis, while the opposite was so with β-subunit mRNA. Differences in the isoenzyme patterns of the two tissues are likely to be due, at least in part, to differences in transcription by the Hexa and Hexb genes.

AB - mRNA for the α- and β-subunits of mouse β-N-acetylhexosaminidase were detected in tissue sections of epididymis and testis using3H-labelled antisense RNA probes by hybridization in situ. There were clear differences in the intensity of the signals given by the two tissues with α-subunit mRNA being more abundant in testis than epididymis, while the opposite was so with β-subunit mRNA. Differences in the isoenzyme patterns of the two tissues are likely to be due, at least in part, to differences in transcription by the Hexa and Hexb genes.

UR - http://www.scopus.com/inward/record.url?scp=0025943051&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025943051&partnerID=8YFLogxK

U2 - 10.1007/BF01041380

DO - 10.1007/BF01041380

M3 - Article

VL - 23

SP - 490

EP - 494

JO - Histochemical Journal

JF - Histochemical Journal

SN - 0018-2214

IS - 10

ER -