Analysis of the tumor-associated antigen TSP-180. Identity with α6-β4 in the integrin superfamily

S. J. Kennel, L. J. Foote, R. Falcioni, A. Sonnenberg, C. D. Stringer, C. Crouse, M. E. Hemler

Research output: Contribution to journalArticlepeer-review


The tumor-associated antigen complex, TSP-180, was previously defined in carcinoma cell lines and found to be expressed in higher amounts in tumor than in normal tissue. Here, the mouse TSP-180 complex is shown to consist of three related proteins (bands 1, 2, and 3) associated with a distinct protein (band 5) that is probably derived from a precursor protein (band 4). All of these proteins are cell surface glycoproteins, and the largest protein (band 1) can be readily labeled with 32PO4. The mouse TSP-180 complex described here strongly resembles the recently described human integrin α64 complex. This homology was confirmed using two distinct rat anti-α6 monoclonal antibodies, each of which recognized both human α64 and mouse TSP-180 complexes. Furthermore, the TSP-180 band 5 protein (mouse α6) had an N-terminal sequence identical to that of human α6. Finally, two different monoclonal antibodies are described, 346-11A and 439-9B, which directly recognize the multiple forms of mouse and human β4 proteins, respectively.

Original languageEnglish
Pages (from-to)15515-15521
Number of pages7
JournalJournal of Biological Chemistry
Issue number26
Publication statusPublished - 1989

ASJC Scopus subject areas

  • Biochemistry


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