Analysis, physiological and clinical significance of 12-HETE: A neglected platelet-derived 12-lipoxygenase product

Research output: Contribution to journalArticle

Abstract

While the importance of cyclooxygenase (COX) in platelet function has been amply elucidated, the identification of the role of 12-lipoxygenase (12-LOX) and of its stable metabolite, 12-hydroxyeicosat. retraenoic acid (12-HETE), has not been clarified as yet. Many studies have analysed the implications of 12-LOX products in different pathological disorders but the information obtained from these works is controversial. Several analytical methods have been developed over the years to simultaneously detect eicosanoids, and specifically 12-HETE, in different biological matrices, essentially enzyme-linked immunosorbent assays (ELISA), radioimmunoassays (RIA), high performance liquid chromatography (HPLC) and mass spectrometry coupled with both gas and liquid chromatography methods (GC- and LC-MS). This review is aimed at summarizing the up to now known physiological and clinical features of 12-HETE together with the analytical methods used for its determination, focusing on the critical issues regarding its measurement.

Original languageEnglish
Pages (from-to)26-40
Number of pages15
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume964
DOIs
Publication statusPublished - Aug 1 2014

Keywords

  • 12-HETE
  • 12-LOX clinical implication
  • Analytical method
  • Arachidonic acid metabolism
  • Platelet

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Cell Biology
  • Clinical Biochemistry
  • Medicine(all)

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