Analytic vaccinology: Antibody-driven design of a human cytomegalovirus subunit vaccine

Anna Kabanova, Daniele Lilleri

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Identification of the most relevant protective antigens has represented a considerable obstacle for the development of subunit vaccines against viral infections, including human cytomegalovirus (HCMV) infection. This chapter describes the method of analytic vaccinology, centered on the clonal analysis of human B cell response to HCMV, which represents an essential tool for assessing the impact of individual viral antigens in the antiviral antibody response. By providing key information on the immunogenicity and protective properties of the antibodies elicited by viral proteins, the analytic vaccinology method guides the selection of the most appropriate vaccine candidates. Here we discuss methodologies for the generation of human monoclonal antibodies from B cells of immune donors, antibody screening in in vitro assays of antigen binding and virus neutralization, and strategies of animal immunization useful for the preclinical evaluation of selected viral antigens. The approach of analytic vaccinology could be universally applied to the characterization of B-cell immune response against any virus of interest and ultimately used for vaccine development.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages167-186
Number of pages20
Volume1403
DOIs
Publication statusPublished - Apr 1 2016

Publication series

NameMethods in Molecular Biology
Volume1403
ISSN (Print)10643745

Keywords

  • Human monoclonal antibodies
  • Neutralizing
  • Non-neutralizing
  • Screening
  • Subunit vaccine
  • Viral antigens
  • Virus

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Kabanova, A., & Lilleri, D. (2016). Analytic vaccinology: Antibody-driven design of a human cytomegalovirus subunit vaccine. In Methods in Molecular Biology (Vol. 1403, pp. 167-186). (Methods in Molecular Biology; Vol. 1403). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3387-7_8