Analytical assessment of MALDI-TOF Imaging Mass Spectrometry on thin histological samples. An insight in proteome investigation

Gianluca Maddalo, Francesca Petrucci, Manuela Iezzi, Tania Pannellini, Piero Del Boccio, Domenico Ciavardelli, Antonino Biroccio, Federica Forlì, Carmine Di Ilio, Enzo Ballone, Andrea Urbani, Giorgio Federici

Research output: Contribution to journalArticlepeer-review

Abstract

Background: The development of MALDI Imaging Mass Spectrometry is a promising technique in the investigation of biological molecular repertoire. We have pursued an analytical assessment of this technique in its application to proteome analysis. Methods: A specific statistical method of analysis has been developed to enable data processing in the absence of internal standards, by defining similarity scores. Results: The investigated linear mode MALDI-TOF set-up allows to obtain data variations comprised within the 30% of variation when assaying tissues samples from the same animal, while the 60% of variation was highlighted in the inter-mice series assaying syngenic animals. Conclusions: This analytical assessment represents the first step of a process that should validate the utilisation of this technique in the clinical practice.

Original languageEnglish
Pages (from-to)210-218
Number of pages9
JournalClinica Chimica Acta
Volume357
Issue number2
DOIs
Publication statusPublished - Jul 24 2005

Keywords

  • Cancer
  • MALDI-TOF
  • Molecular imaging
  • Molecular markers
  • Proteomics

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

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