Analytical evaluation of the novel helena V8 capillary electrophoresis system

Giuseppe Lippi, Luisita Battistelli, Arialdo Vernocchi, Michele Mussap

Research output: Contribution to journalArticle


Background: Capillary electrophoresis (CE) is used for the screening of protein abnormalities in serum and other biological fluids. Since the Helena V8 CE system has been recently commercialized, the aim of this study was to assess its analytical performance under routine laboratory conditions. Methods: The evaluation of Helena V8 (Medical Systems S.p.A., Genova, Italy) for protein fractions (i.e., albumin, alpha-1, alpha-2, beta-1, beta-2, gamma, albumin/globulin ratio [A/G]) and monoclonal component (MC) quantification included imprecision studies, linearity, and comparison with Sebia Capillarys (Sebia, Evry Cedex, France), which was considered the reference analyzer. Results: The imprecision of Helena V8 on all parameters was comprised between 0.8% and 15.2%; the linearity was excellent up to an MC value of 14.5% (r=1.00). The comparison studies showed excellent Spearman's correlations with Sebia Capillarys, with coefficients comprised between 0.84 and 0.99 (all p

Original languageEnglish
Pages (from-to)245-249
Number of pages5
JournalJournal of Medical Biochemistry
Issue number3
Publication statusPublished - Jun 2013



  • Analytical evaluation
  • Capillary electrophoresis
  • Protein separation

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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