L’interferenza dell’emolisi sulla misura dell’insulina

Translated title of the contribution: Analytical interference of hemolysis on serum insulin measurement.

Federica de Liso, Loredana Maldari, Paola de Corato, Mattia Speroni, Rita Maiavacca, Ferruccio Ceriotti

Research output: Contribution to journalArticlepeer-review

Abstract

Insulin is a polypeptide hormone, secreted from pancreatic β-cell, involved in the regulation of glucose and lipid metabolism. Insulin measurement is a useful tool to identify some clinical conditions, such as fasting hypoglycemia, some types of diabetes, the presence of insulin resistance. An important pre-analytical aspect that influences the determination of insulin serum concentration is the presence of hemolysis. In fact, it is well known that insulin is degraded by a protease released by red blood cells after hemolysis. The aim of this study is to evaluate the interference of hemolysis on insulin measurements performed by a chemiluminescence method. To study the effect of hemolysis on insulin degradation, we added increasing concentrations of red blood cell hemolysate to a serum pool with known insulin concentration. The reduction of insulin levels was affected by the degree of hemolysis, by the time elapsed before the assay and by the temperature of samples storage. Our results show that insulin values do not decrease significantly (<10%) when hemolysis is <2.0 g/L of free hemoglobin (corresponding to H-index=200) if samples are maintained at low temperatures (i.e. in an ice-water slurry) until the assay is performed.

Translated title of the contributionAnalytical interference of hemolysis on serum insulin measurement.
Original languageItalian
JournalBiochimica Clinica
Volume43
Issue number3
DOIs
Publication statusPublished - Jan 1 2019

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Medical Laboratory Technology
  • Biochemistry, medical

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