Celiac disease (CD) is an autoimmune enteropathy characterized by intestinal malabsorption and immunological responses to dietary gliadins and an auto antigen located in the endomysium. The latter has recently been identified as the enzyme tissue transglutaminase (tTG). The linkage between gliadins, tTG and the autoimmune response has still to be clarified. In this work we report the production and analysis of a phage antibody library from the peripheral blood lymphocytes (PLB) of a CD patient. The library contained polyreactive and monoreactive antibodies to α-gliadin, to the dietary antigen β-lactoglobulin, but not to tTG. The majority of the VHH regions of the anti-α-gliadin antibodies belonged to the VH 4 family. The possibility of exploiting phage display antibodies as tools to study the molecular events associated with CD is discussed.
|Number of pages||7|
|Publication status||Published - 2000|
- α-gliadin, human tissue transglutaminase
- Antibody phage library
- Celiac disease
ASJC Scopus subject areas